Application | Comment | Organism |
---|---|---|
analysis | detection of localized extracellular sites of protease activity by use of fluorescent biosensor rhodamine 6G-labeled KDP-6-aminohexanoic acid-GPLGIAGIG-6-aminohexanoic acid-PKGY. Protease activity is localized at the polarized leading edge of migrating tumor cells rather than further back on the cell body. The path of proteolytic cleavage by a migrating cell can be visualized in 2- and 3-dimensional matrices. Probe can be used to determine inhibitor concentrations needed to suppress cell-surface protease activity | Homo sapiens |
medicine | protease activity is localized at the polarized leading edge of migrating tumor cells rather than further back on the cell body. Activity is essential for cell migration in native cross-linked but not pepsin-treated collagen matrices | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: tissue inhibitor of matrix metalloproteinase-1 | Homo sapiens | |
tissue inhibitor of matrix metalloproteinase-2 | 0.1 microM, inhibits both protease activity and migration in a 3-dimensional cross-linked collagen matrix | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HT-1080 cell | - |
Homo sapiens | - |
PC-3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rhodamine 6G-labeled KDP-6-aminohexanoic acid-GPLGIAGIG-6-aminohexanoic acid-PKGY + H2O | fluorescent biosensor, substrate for matrix metalloproteinases MMP-2, MMP-9, MMP-14 | Homo sapiens | rhodamine 6G-labeled KDP-6-aminohexanoic acid-GPLG + IAGIG-6-aminohexanoic acid-PKGY | - |
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