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Literature summary for 3.4.24.69 extracted from

  • Benoit, R.; Frey, D.; Hilbert, M.; Kevenaar, J.; Wieser, M.; Stirnimann, C.; McMillan, D.; Ceska, T.; Lebon, F.; Jaussi, R.; Steinmetz, M.; Schertler, G.; Hoogenraad, C.; Capitani, G.; Kammerer, R.
    Structural basis for recognition of synaptic vesicle protein 2C by botulinum neurotoxin A (2014), Nature, 505, 108-111.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli NiCo21(DE3) cells Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment) Organism
botulinum neurotoxin A-receptor-binding domain in complex with the SV2C luminal domain, vapor diffusion method, using 100 mM HEPES, pH 7.5, 6% (w/v) PEG 8000, 8% (v/v) glycerol and 100 mM NaCl Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Neuroexocytosis multi-subunit complex + H2O Clostridium botulinum
-
 ?
-
 ?
SNAP-25 + H2O Clostridium botulinum
-
 ?
-
 ?
synaptobrevin + H2O Clostridium botulinum
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum P10845
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity chromatography or GST Sepharose affinity chromatography followed by Superdex 200 gel filtration Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Neuroexocytosis multi-subunit complex + H2O
-
 Clostridium botulinum ?
-
 ?
SNAP-25 + H2O
-
 Clostridium botulinum ?
-
 ?
synaptobrevin + H2O
-
 Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/A subtype Clostridium botulinum
botulinum neurotoxin a
-
 Clostridium botulinum