Literature summary for 3.4.24.69 extracted from

Zhang, Y.; Gardberg, A.; Edwards, T.; Sankaran, B.; Robinson, H.; Varnum, S.; Buchko, G.
Structural insights into the functional role of the Hcn sub-domain of the receptor-binding domain of the botulinum neurotoxin mosaic serotype C/D (2013), Biochimie, 95, 1379-1385.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment)	Organism
subtype BoNT/CD-heavy chain receptor binding domain, hanging drop vapor diffusion method, using 1.6 M (NH4)2SO4, 2% (w/v) PEG 400, and 0.1 M HEPES, pH 7.5	Clostridium botulinum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	1 * 100000 + 1 * 50000, SDS-PAGE	Clostridium botulinum
100000	-	1 * 100000 + 1 * 50000, SDS-PAGE	Clostridium botulinum

Organism

Organism	UniProt	Comment	Textmining
Clostridium botulinum	Q5DW55	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Clostridium botulinum

Subunits

Subunits	Comment	Organism
heterodimer	1 * 100000 + 1 * 50000, SDS-PAGE	Clostridium botulinum

Synonyms

Synonyms	Comment	Organism
BoNT/CD	subtype	Clostridium botulinum
Botulinum neurotoxin	-	Clostridium botulinum

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Release 2023.1 (January 2023)