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Literature summary for 3.4.24.69 extracted from

  • Kumar, R.; Kukreja, R.; Cai, S.; Singh, B.
    Differential role of molten globule and protein folding in distinguishing unique features of botulinum neurotoxin (2014), Biochim. Biophys. Acta, 1844, 1145-1152.
    View publication on PubMed
Search for more literature for 3.4.24.69

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 100000 + 1 * 50000, SDS-PAGE Clostridium botulinum
100000
-
 1 * 100000 + 1 * 50000, SDS-PAGE Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum P10844
-
-
Clostridium botulinum Q00496
-
-
Clostridium botulinum Q7B8V4
-
-

Purification (Commentary)

Purification (Comment) Organism
the light chain is purified by nickel affinity column chromatography Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SNAPEtide + H2O substrate for subtype BoNT/E Clostridium botulinum ?
-
 ?
VAMPTide + H2O substrate for subtype BoNT/B Clostridium botulinum ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 100000 + 1 * 50000, SDS-PAGE Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT/A subtype Clostridium botulinum
BoNT/B subtype Clostridium botulinum
BoNT/E subtype Clostridium botulinum
Botulinum neurotoxin
-
 Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Clostridium botulinum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50 the light chain of subtype BoNT/B loses its activity beyond 37°C, with total loss at 50°C Clostridium botulinum
37 50 the light chain of subtype BoNT/E loses its activity beyond 37°C but remains active even up to 50°C, retaining about 44% activity Clostridium botulinum