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Literature summary for 3.4.24.69 extracted from

  • Lee, K.; Lam, K.; Kruel, A.; Perry, K.; Rummel, A.; Jin, R.
    High-resolution crystal structure of HA33 of botulinum neurotoxin type B progenitor toxin complex (2014), Biochem. Biophys. Res. Commun., 446, 568-573.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-RIL (DE3) cells Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment) Organism
hemagglutinin 33 component of botulinum neurotoxin type B progenitor toxin complex bound to lactose, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.0), 5% MPD, 5% (w/v) PEG [poly(ethylene glycol)] 6k, and 20 mM lactose Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
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-
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Clostridium botulinum Okra
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-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT
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 Clostridium botulinum
botulinum neurotoxin type B
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 Clostridium botulinum

General Information

General Information Comment Organism
physiological function Botulinum neurotoxins are produced as progenitor toxin complexes by Clostridium botulinum. The progenitor toxin complexes are composed of enzyme and non-toxic neurotoxin-associated proteins (NAPs), which serve to protect and deliver the enzyme through the gastrointestinal tract in food borne botulism. HA33 is a key NAP component that specifically recognizes host carbohydrates and helps enrich progenitor toxin complex on the intestinal lumen preceding its transport across the epithelial barriers Clostridium botulinum