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Literature summary for 3.4.24.69 extracted from
- Holotoxin activity of botulinum neurotoxin subtype A4 originating from a nontoxigenic Clostridium botulinum expression system (2014), Appl. Environ. Microbiol., 80, 7415-7422.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in nonsporulating and nontoxigenic Clostridium botulinum expression host strain | Clostridium botulinum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L260F/I264R | these mutations do not alter the activity of the enzyme | Clostridium botulinum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the recombinant subtype A4 neurotoxin is effectively neutralized by botulism heptavalent antitoxin | Clostridium botulinum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
x * 150000, SDS-PAGE | Clostridium botulinum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | Clostridium botulinum | - |
? | - |
? |  |
| SNAP-25 + H2O | Clostridium botulinum 657Ba | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
| Clostridium botulinum 657Ba | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Ni-NTA agarose column chromatography, and DEAE-Sephadex gel filtration | Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | - |
Clostridium botulinum | ? | - |
? | |
| SNAP-25 + H2O | - |
Clostridium botulinum 657Ba | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 150000, SDS-PAGE | Clostridium botulinum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/A4 | - |
Clostridium botulinum |
| Botulinum neurotoxin | - |
Clostridium botulinum |
| subtype A4 neurotoxin | - |
Clostridium botulinum |
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Release 2023.1 (January 2023)
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