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Literature summary for 3.4.24.69 extracted from
- Production of catalytically inactive BoNT/A1 holoprotein and comparison with BoNT/A1 subunit vaccines against toxin subtypes A1, A2, and A3 (2009), Vaccine, 27, 4490-4497.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | evaluation of catalytically inactive BoNT/A1 mutant H223A/E224A/H227A holoprotein as a vaccine candidate by comparison against recombinant BoNT/A1 LC, LC-belt, LC-Hn, and Hc antigens and a LC-Hn +Hc combination in mouse potency and efficacy bioassays when challenged with BoNT/A subtypes /A1, /A2, and /A3, overview | Clostridium botulinum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of catalytically inactive BoNT/A1 mutant H223A/E224A/H227A holoprotein in Pichia pastoris | Clostridium botulinum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H223A/E224A/H227A | site-directed mutagenesis of active site residues, catalytically inactive BoNT/A1 mutant | Clostridium botulinum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required | Clostridium botulinum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | Clostridium botulinum | i.e. 25-kDa synaptosome-associated protein | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
BoNT/A1 complex from Hall strain, BoNT/A2 complex from FRI-honey strain, and BoNT/A3 complex from Loch Maree strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant catalytically inactive BoNT/A1 mutant H223A/E224A/H227A holoprotein from Pichia pastoris by cation exchange chromatography to about 95% homogeneity. The purified ciBoNT/A1 HP is a mixed population of singlechain and nicked dichain | Clostridium botulinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Clostridium botulinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.8 | - |
69nM purified native BoNT/A1 toxin, substrate SNAP25, pH 7.3, 37°C | Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SNAP-25 + H2O | i.e. 25-kDa synaptosome-associated protein | Clostridium botulinum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | BoNTs consist of a light chain of 50 kDa and a heavy chain of 100 kDa | Clostridium botulinum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/A1 | - |
Clostridium botulinum |
| Clostridium botulinum neurotoxin A1 | - |
Clostridium botulinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Clostridium botulinum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Clostridium botulinum | recombinant catalytically inactive BoNT/A1 mutant H223A/E224A/H227A holoprotein, sequence calculation | - |
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