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Literature summary for 3.4.24.69 extracted from

  • Stowe, G.N.; Silhar, P.; Hixon, M.S.; Silvaggi, N.R.; Allen, K.N.; Moe, S.T.; Jacobson, A.R.; Barbieri, J.T.; Janda, K.D.
    Chirality holds the key for potent inhibition of the botulinum neurotoxin serotype A protease (2010), Org. Lett., 12, 756-759.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Inhibitors

Inhibitors Comment Organism Structure
(2E)-3-(2,4-dichlorophenyl)-N-hydroxyprop-2-enamide
-
 Clostridium botulinum
(2E)-3-(4-chlorophenyl)-N-hydroxyprop-2-enamide a trans-cinnamic hydroxamate Clostridium botulinum
(3R)-3-(2,4-dichlorophenyl)-N,5-dihydroxypentanamide
-
 Clostridium botulinum
(3R)-3-(4-chlorophenyl)-N,5-dihydroxypentanamide
-
 Clostridium botulinum
(3S)-3-(2,4-dichlorophenyl)-N,5-dihydroxypentanamide
-
 Clostridium botulinum
(3S)-3-(4-chlorophenyl)-N,5-dihydroxypentanamide
-
 Clostridium botulinum
3-(4-chloro-2-methylphenyl)-N-hydroxypropanamide
-
 Clostridium botulinum
additional information inhibitor synthesis, overview Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium botulinum the toxin light chain, LC, is a zinc-dependent endopeptidase that cleaves soluble N-ethylmaleimide-sensitive fusion proteins, SNARE, located at nerve endings ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the toxin light chain, LC, is a zinc-dependent endopeptidase that cleaves soluble N-ethylmaleimide-sensitive fusion proteins, SNARE, located at nerve endings Clostridium botulinum ?
-
 ?
SNAP-25 + H2O i.e. 25-kDa synaptosome-associated protein, truncated version of SNAP-25 Clostridium botulinum ?
-
 ?

Subunits

Subunits Comment Organism
More BoNTs are synthesized as150-kDa proteins consisting of a 100-kDa heavy chain, HC, and a 50-kDa light chain, LC, linked by disulfide bonds Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT/A
-
 Clostridium botulinum
botulinum neurotoxin serotype A
-
 Clostridium botulinum
botulinum neurotoxin serotype A protease
-
 Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22.5
-
 assay at Clostridium botulinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Clostridium botulinum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00016
-
 (3R)-3-(2,4-dichlorophenyl)-N,5-dihydroxypentanamide pH 7.4, 22.5°C Clostridium botulinum
0.0017
-
 (3R)-3-(4-chlorophenyl)-N,5-dihydroxypentanamide pH 7.4, 22.5°C Clostridium botulinum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0004
-
 pH 7.4, 22.5°C Clostridium botulinum (2E)-3-(2,4-dichlorophenyl)-N-hydroxyprop-2-enamide
0.001
-
 pH 7.4, 22.5°C Clostridium botulinum (3R)-3-(2,4-dichlorophenyl)-N,5-dihydroxypentanamide
0.003
-
 pH 7.4, 22.5°C Clostridium botulinum 3-(4-chloro-2-methylphenyl)-N-hydroxypropanamide
0.008
-
 pH 7.4, 22.5°C Clostridium botulinum (3R)-3-(4-chlorophenyl)-N,5-dihydroxypentanamide
0.015
-
 pH 7.4, 22.5°C Clostridium botulinum (2E)-3-(4-chlorophenyl)-N-hydroxyprop-2-enamide
0.021
-
 pH 7.4, 22.5°C Clostridium botulinum (3S)-3-(2,4-dichlorophenyl)-N,5-dihydroxypentanamide
0.036
-
 pH 7.4, 22.5°C Clostridium botulinum (3S)-3-(4-chlorophenyl)-N,5-dihydroxypentanamide