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Literature summary for 3.4.24.69 extracted from

  • Shi, X.; Garcia, G.E.; Neill, R.J.; Gordon, R.K.
    TCEP treatment reduces proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells (2009), J. Cell. Biochem., 107, 1021-1030.
    View publication on PubMed
Search for more literature for 3.4.24.69

Activating Compound

Activating Compound Comment Organism Structure
tris-(2-carboxyethyl)-phosphine hydrochloride i.e. TCEP, a non-odorous, oxygen-insensitive, non-toxic sulfhydryl reducing compound, activates proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells maximally at 1mM, protects against BoNT/B inhibition of noradrenaline release, achieving 72% of the release from un-intoxicated controls. TCEP significantly changes the conformation of BoNT/B holotoxin Clostridium botulinum

Inhibitors

Inhibitors Comment Organism Structure
tris-(2-carboxyethyl)-phosphine hydrochloride i.e. TCEP, a non-odorous, oxygen-insensitive, non-toxic sulfhydryl reducing compound, reduces proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells at higher concentrations above 4 mM, protects against BoNT/B inhibition of noradrenaline release, achieving 72% of the release from un-intoxicated controls. TCEP significantly changes the conformation of BoNT/B holotoxin. But TCEP does not fragment un-nicked BoNT/B holotoxin Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc-dependent endopeptidase Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
VAMP2 + H2O Clostridium botulinum i.e. synaptobrevin-2 or vesicle-associated membrane protein 2 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information assay method measuring noradrenaline release in human neuronal SHSY-5Y cells Clostridium botulinum ?
-
 ?
VAMP2 + H2O i.e. synaptobrevin-2 or vesicle-associated membrane protein 2 Clostridium botulinum ?
-
 ?
VAMP2 + H2O i.e. vesicle-associated membrane protein 2 or synaptobrevin-2, with BoNT/B light chain. BoNT/B HT exhibits little ability to cleave its substrate VAMP-2, when its LC and HC subunits are held together by a disulfide bond Clostridium botulinum ?
-
 ?
VAMPTide + H2O a VAMP-2-derived peptide substrate, modified with FRET, with BoNT/B light chain Clostridium botulinum ?
-
 ?

Subunits

Subunits Comment Organism
More the BoNT HC-LC subunits are held together by a single disulfide bond Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT/B
-
 Clostridium botulinum
botulinum neurotoxin B
-
 Clostridium botulinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Clostridium botulinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Clostridium botulinum

General Information

General Information Comment Organism
additional information the catalytic light chain, LC, of botulinum neurotoxin B is unable to enter target neuronal cells by itself. It is brought into the cell, human neuronal SHSY-5Y cells, in association with the BoNT/B heavy chain, HC, through endocytosis Clostridium botulinum