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Literature summary for 3.4.24.69 extracted from

  • Schmitt, J.; Karalewitz, A.; Benefield, D.A.; Mushrush, D.J.; Pruitt, R.N.; Spiller, B.W.; Barbieri, J.T.; Lacy, D.B.
    Structural analysis of botulinum neurotoxin type G receptor binding (2010), Biochemistry, 49, 5200-5205.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant His-tagged and/or FLAG-tagged heavy chains, BoNT/G HCR, BoNT/A HCR, and BoNT/B HCR, in Escherichia coli strain BL21(DE3) Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged BoNT/G HCR, hanging drop vapour diffusion method, 11.5 mg/mL protein in mother liquor containing 12-15% w/v PEG 3350, 20 mM Bis-Tris buffer, pH 5.75-6.5, and 20-25 mM MgCl, X-ray diffraction structure determination and analysis at 2.0 A resolution, modelling Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium botulinum botulinum neurotoxin binds host peripheral neurons at the neuromuscular junction through a dual-receptor mechanism that includes interactions with ganglioside and protein receptors. The receptor identities vary depending on BoNT serotype. BoNT/B and BoNT/G bind the luminal domains of synaptotagmin I and II, homologous synaptic vesicle proteins, structure analysis of BoNT/G binding to Syt andGT1b, overview ?
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Organism

Organism UniProt Comment Textmining
Clostridium botulinum Q60393 BoNT serotypes A-G
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged and/or FLAG-tagged heavy chains, BoNT/G HCR, BoNT/A HCR, and BoNT/B HCR, from Escherichia coli by nickel affinity chromatography and gel filtration Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information botulinum neurotoxin binds host peripheral neurons at the neuromuscular junction through a dual-receptor mechanism that includes interactions with ganglioside and protein receptors. The receptor identities vary depending on BoNT serotype. BoNT/B and BoNT/G bind the luminal domains of synaptotagmin I and II, homologous synaptic vesicle proteins, structure analysis of BoNT/G binding to Syt andGT1b, overview Clostridium botulinum ?
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Subunits

Subunits Comment Organism
More structure comparisons of BoNT/G HCR, BoNT/A HCR, and BoNT/B HCR, modelling, overview Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT
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Clostridium botulinum
Botulinum neurotoxin
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Clostridium botulinum