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Literature summary for 3.4.24.69 extracted from

  • Nuemket, N.; Tanaka, Y.; Tsukamoto, K.; Tsuji, T.; Nakamura, K.; Kozaki, S.; Yao, M.; Tanaka, I.
    Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum (2010), Acta Crystallogr. Sect. F, 66, 608-610.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the C-terminally His-tagged BoNT/OFD05 receptor-binding domain, wild-type and SeMet-labeled, in Escherichia coli strain B843 (DE3) Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant native and SeMet-derivative enzyme, mixing of 0.001 ml protein solution containing 1 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 200 mM NaCl, with 0.001 ml reservoir buffer, containing 0.2 M potassium/sodium tartrate, 0.1 M trisodium citrate, pH 5.6, and 1 M ammonium sulfate for the native enzyme, and 0.1 M MES pH 6.5, 1.6 M magnesium sulfate, and 1 M sodium chloride for the selenium methionine-labeled enzyme, equilibration against 0.1 ml reservoir buffer, 20°C, X-ray diffraction structure determination and analysis at 2.8 A and 3.1 A resolution, respectively Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-dependent endopeptidase Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum A5JGM8 strain OFD05, isolated from bovine botulism, seven botulinum toxin serotypes, BoNTs, designated A-G
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Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged wild-type and SeMet-labeled BoNT/OFD05 receptor-binding domain from Escherichia coli strain B843 (DE3) by nickel affinity chromatography, dialysis, and gel filtration Clostridium botulinum

Source Tissue

Source Tissue Comment Organism Textmining
additional information serotype BoNT/C requires a single ganglioside as a binding receptor on neuronal cells, while serotypes BoNT/A and BoNT/B require two receptors for specific binding. Binding mechanism of the BoNT/OFD05 binding domain and its ganglioside receptors on neuronal host cells, overview Clostridium botulinum
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Subunits

Subunits Comment Organism
More BoNTs are large neurotoxic proteins of about 150 kDa that consist of a light chain of 50 kDa, and a heavy chain of 100 kDa linked by a disulfide bond. BoNTs possess binding, translocation and catalytic domains Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT
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Clostridium botulinum
botulinum toxin
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Clostridium botulinum
More BoNT from Clostridium botulinum OFD05, isolated from bovine botulism in Japan, is a D/C mosaic-type BoNT Clostridium botulinum