Cloned (Comment) | Organism |
---|---|
recombinant expression of the C-terminally His-tagged BoNT/OFD05 receptor-binding domain, wild-type and SeMet-labeled, in Escherichia coli strain B843 (DE3) | Clostridium botulinum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant native and SeMet-derivative enzyme, mixing of 0.001 ml protein solution containing 1 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 200 mM NaCl, with 0.001 ml reservoir buffer, containing 0.2 M potassium/sodium tartrate, 0.1 M trisodium citrate, pH 5.6, and 1 M ammonium sulfate for the native enzyme, and 0.1 M MES pH 6.5, 1.6 M magnesium sulfate, and 1 M sodium chloride for the selenium methionine-labeled enzyme, equilibration against 0.1 ml reservoir buffer, 20°C, X-ray diffraction structure determination and analysis at 2.8 A and 3.1 A resolution, respectively | Clostridium botulinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-dependent endopeptidase | Clostridium botulinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | A5JGM8 | strain OFD05, isolated from bovine botulism, seven botulinum toxin serotypes, BoNTs, designated A-G | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged wild-type and SeMet-labeled BoNT/OFD05 receptor-binding domain from Escherichia coli strain B843 (DE3) by nickel affinity chromatography, dialysis, and gel filtration | Clostridium botulinum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | serotype BoNT/C requires a single ganglioside as a binding receptor on neuronal cells, while serotypes BoNT/A and BoNT/B require two receptors for specific binding. Binding mechanism of the BoNT/OFD05 binding domain and its ganglioside receptors on neuronal host cells, overview | Clostridium botulinum | - |
Subunits | Comment | Organism |
---|---|---|
More | BoNTs are large neurotoxic proteins of about 150 kDa that consist of a light chain of 50 kDa, and a heavy chain of 100 kDa linked by a disulfide bond. BoNTs possess binding, translocation and catalytic domains | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
BoNT | - |
Clostridium botulinum |
botulinum toxin | - |
Clostridium botulinum |
More | BoNT from Clostridium botulinum OFD05, isolated from bovine botulism in Japan, is a D/C mosaic-type BoNT | Clostridium botulinum |