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Literature summary for 3.4.24.69 extracted from
- Structural and biochemical studies of botulinum neurotoxin serotype C1 light chain protease: implications for dual substrate specificity (2007), Biochemistry, 46, 10685-10693.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally His-tagged BoNT/C1-LC in Escherichia coli strain M15[pREP4] | Clostridium botulinum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant His-tagged BoNT/C1-LC, vapour diffusion method, 12 mg/ml protein in 20 mM HEPES, pH 7.4, is mixed with an equal volume of reservoir solution containing 1.6 M sodium formate and 0.1 M sodium citrate, pH 4.6-5.0, 4°C, cryoprotection in the same mother liquor supplemented with 20% glycerol, X-ray structure determination and analysis at 1.75 A resolution, molecular replacement | Clostridium botulinum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | the catalytic component of the clostridial neurotoxins is their light chain, a Zn2+ endopeptidase | Clostridium tetani |  |
| Zn2+ | the catalytic component of the clostridial neurotoxins is their light chain, a Zn2+ endopeptidase | Clostridium botulinum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Clostridium tetani | clostridial neurotoxins are the causative agents of the neuroparalytic diseases botulism and tetanus blocking neurotransmitter release through specific proteolysis of one of the three soluble N-ethylmaleimide-sensitive-factor attachment protein receptors, SNAP-25, syntaxin, and synaptobrevin, which constitute part of the synaptic vesicle fusion machinery | ? | - |
? | |
| additional information | Clostridium botulinum | clostridial neurotoxins are the causative agents of the neuroparalytic diseases botulism and tetanus blocking neurotransmitter release through specific proteolysis of one of the three soluble N-ethylmaleimide-sensitive-factor attachment protein receptors, SNAP-25, syntaxin, and synaptobrevin, which constitute part of the synaptic vesicle fusion machinery | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
serotypes A to G | - |
| Clostridium tetani | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged BoNT/C1-LC from Escherichia coli strain M15[pREP4] by nickel affinity chromatography | Clostridium botulinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | clostridial neurotoxins are the causative agents of the neuroparalytic diseases botulism and tetanus blocking neurotransmitter release through specific proteolysis of one of the three soluble N-ethylmaleimide-sensitive-factor attachment protein receptors, SNAP-25, syntaxin, and synaptobrevin, which constitute part of the synaptic vesicle fusion machinery | Clostridium tetani | ? | - |
? | |
| additional information | clostridial neurotoxins are the causative agents of the neuroparalytic diseases botulism and tetanus blocking neurotransmitter release through specific proteolysis of one of the three soluble N-ethylmaleimide-sensitive-factor attachment protein receptors, SNAP-25, syntaxin, and synaptobrevin, which constitute part of the synaptic vesicle fusion machinery | Clostridium botulinum | ? | - |
? | |
| additional information | the catalytic component of the clostridial neurotoxins is their light chain, a Zn2+ endopeptidase, active site structure of serotype C1, substrate and cleavage site specificity of serotypes, overview | Clostridium botulinum | ? | - |
? | |
| additional information | the catalytic component of the clostridial neurotoxins is their light chain, a Zn2+ endopeptidase, cleavage site specificity, overview | Clostridium tetani | ? | - |
? | |
| SNAP-25 + H2O | - |
Clostridium tetani | ? | - |
? | |
| SNAP-25 + H2O | serotype BoNT/C1-LC exhibits dual specificity toward both syntaxin and SNAP-25, in contrast to other serotypes, due to a distinct pocket S1' near the active site likely achieves the correct register for the cleavage site by only allowing Ala as the P1' residue for both SNAP-25 and syntaxin, activity of the serotype C enzyme BoNT/C1-LC with diverse SNAp-25 substrate mutants, overview | Clostridium botulinum | ? | - |
? | |
| synaptobrevin + H2O | - |
Clostridium tetani | ? | - |
? | |
| synaptobrevin + H2O | - |
Clostridium botulinum | ? | - |
? | |
| Syntaxin + H2O | - |
Clostridium tetani | ? | - |
? | |
| Syntaxin + H2O | serotype BoNT/C1-LC exhibits dual specificity toward both syntaxin and SNAP-25, in contrast to other serotypes, due to a distinct pocket S1' near the active site likely achieves the correct register for the cleavage site by only allowing Ala as the P1' residue for both SNAP-25 and syntaxin | Clostridium botulinum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT | - |
Clostridium botulinum |
| BoNT/C1-LC | - |
Clostridium botulinum |
| Botulinum neurotoxin | - |
Clostridium botulinum |
| botulinum neurotoxin serotype C1 light chain protease | - |
Clostridium botulinum |
| Tetanus neurotoxin | - |
Clostridium tetani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Clostridium botulinum |
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