Literature summary for 3.4.24.69 extracted from

Arndt, J.W.; Chai, Q.; Christian, T.; Stevens, R.C.
Structure of botulinum neurotoxin type D light chain at 1.65 A resolution: repercussions for VAMP-2 substrate specificity (2006), Biochemistry, 45, 3255-3262.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
nanodroplet vapor diffusion method, 1.65 A resolution crystal structure of the catalytic domain of BoNT serotype D light chain. Structural analysis has identified a hydrophobic pocket potentially involved in substrate recognition of the P1' VAMP residue (Leu 60) and a second remote site for recognition of the V1 SNARE motif that is critical for activity. A structural comparison of BoNT/D-LC with BoNT/F-LC that also recognizes VAMP-2 one residue away from the BoNT/D-LC site provides additional molecular details about the unique serotype specific activities. In particular, BoNT/D prefers a hydrophobic interaction for the V1 motif of VAMP-2, while BoNT/F adopts a more hydrophilic strategy for recognition of the same V1 motif	Clostridium botulinum

Crystallization (Comment)

Organism

nanodroplet vapor diffusion method, 1.65 A resolution crystal structure of the catalytic domain of BoNT serotype D light chain. Structural analysis has identified a hydrophobic pocket potentially involved in substrate recognition of the P1' VAMP residue (Leu 60) and a second remote site for recognition of the V1 SNARE motif that is critical for activity. A structural comparison of BoNT/D-LC with BoNT/F-LC that also recognizes VAMP-2 one residue away from the BoNT/D-LC site provides additional molecular details about the unique serotype specific activities. In particular, BoNT/D prefers a hydrophobic interaction for the V1 motif of VAMP-2, while BoNT/F adopts a more hydrophilic strategy for recognition of the same V1 motif

Clostridium botulinum

Organism

Organism	UniProt	Comment	Textmining
Clostridium botulinum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Clostridium botulinum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
vesicle-associated membrane protein VAMP-2 + H2O	-	Clostridium botulinum	?	-	?

Synonyms

Synonyms	Comment	Organism
BoNT/D	-	Clostridium botulinum
botulinum neurotoxin type D	-	Clostridium botulinum

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Release 2023.1 (January 2023)