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Literature summary for 3.4.24.68 extracted from

  • Indrawattana, N.; Sookrung, N.; Kulkeaw, K.; Seesuay, W.; Kongngoen, T.; Chongsa-nguan, M.; Tungtrongchitr, A.; Chaicumpa, W.
    Human monoclonal ScFv that inhibits cellular entry and metalloprotease activity of tetanus neurotoxin (2010), Asian Pac. J. Allergy Immunol., 28, 85-93.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information design, random selection, and recombinant production from phagemid in Escherichia coli of human single chain antibody fragments that inhibit tetanus toxin binding to retinoic acid pulsed human neuroblastoma cells and TeNT protease activity completely, overview Clostridium tetani

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Clostridium tetani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
synaptobrevin + H2O Clostridium tetani TeNT is a zinc metalloprotease, that is produced by anaerobically grown Clostridium tetani in infected tissue, where it binds to ganglioside receptors of peripheral nerves. TeNT is then endocytosed. The A subunit exits from the endosome and undergoes retrograde transport via the nerve axon to the spinal cord of the host, where it specifically cleaves one of the nerve cell SNARE proteins, synaptobrevin ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium tetani
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
synaptobrevin + H2O TeNT is a zinc metalloprotease, that is produced by anaerobically grown Clostridium tetani in infected tissue, where it binds to ganglioside receptors of peripheral nerves. TeNT is then endocytosed. The A subunit exits from the endosome and undergoes retrograde transport via the nerve axon to the spinal cord of the host, where it specifically cleaves one of the nerve cell SNARE proteins, synaptobrevin Clostridium tetani ?
-
?
synaptobrevin + H2O a host nerve cell SNARE protein, purified recombinant His-tagged synaptobrevin expressed in Escherichia coli Clostridium tetani ?
-
?

Subunits

Subunits Comment Organism
More TeNT is an A-B toxin. It consits of a heavy chain containing cellular receptor binding domain and a light chain with zinc metalloprotease activity Clostridium tetani

Synonyms

Synonyms Comment Organism
TeNT
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Clostridium tetani
tetanospasmin
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Clostridium tetani
Tetanus neurotoxin
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Clostridium tetani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Clostridium tetani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Clostridium tetani

General Information

General Information Comment Organism
physiological function cleavage of synaptobrevin results in inhibition of release of neurotransmitters glycine and gamma-amino butyric acid from inhibitory interneurons causing spastic paralysis Clostridium tetani