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Literature summary for 3.4.24.68 extracted from

  • Tonello, F.; Pellizzari, R.; Pasqualato, S.; Grandi, G.; Peggion, E.; Montecucco, C.
    Recombinant and truncated tetanus neurotoxin light chain: cloning, expression, purification, and proteolytic activity (1999), Protein Expr. Purif., 15, 221-227.
    View publication on PubMed

Application

Application Comment Organism
medicine very powerful neurotoxin, agent responsible for all clinical symptoms of tetanus Clostridium tetani

Cloned(Commentary)

Cloned (Comment) Organism
tetanus neurotoxin light chain, expressed in Escherichia coli Clostridium tetani

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals obtained with the hanging drop method Clostridium tetani

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc endopeptidase Clostridium tetani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
activated by proteolytic cleavage with formation of a 50 kDa L-chain and a 100 kDa H-chain Clostridium tetani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium tetani most powerful known natural toxin ?
-
?
additional information Clostridium tetani Y-IV-3 most powerful known natural toxin ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium tetani
-
-
-
Clostridium tetani Y-IV-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
tetanus neurotoxin light chain Clostridium tetani

Storage Stability

Storage Stability Organism
4°C, both native and recombinant TeNT L-chains show significant decreases after 3-4 days of storage Clostridium tetani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information most powerful known natural toxin Clostridium tetani ?
-
?
additional information most powerful known natural toxin Clostridium tetani Y-IV-3 ?
-
?
vesicle-associated membrane protein VAMP + H2O L-chain highly specific for the substrate Clostridium tetani ?
-
?
vesicle-associated membrane protein VAMP + H2O L-chain highly specific for the substrate Clostridium tetani Y-IV-3 ?
-
?

Synonyms

Synonyms Comment Organism
TeNT
-
Clostridium tetani
Tetanus neurotoxin
-
Clostridium tetani