Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Proteases | activation by rapid cleavage within an exposed loop of the single inactive MW 150000 polypeptide chain and generation of active di-chain neurotoxin | Clostridium tetani | |
Proteases | bacterial | Clostridium tetani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaOCl | inactivation | Clostridium tetani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | accumulates until bacterial lysis | Clostridium tetani | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc-dependent endoproteinase | Clostridium tetani | |
Zinc | L-chain: form of zinc-endopeptidase, 0.8-1 gatom zinc/mol toxin, bound to light or L-chain | Clostridium tetani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150700 | - |
Clostridium tetani, calculated from amino acid sequence | Clostridium tetani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
synaptobrevin + H2O | Clostridium tetani | enzyme disables neuroexocytosis apparatus, acts at spinal inhibitory interneurons, blocking release of various neurotransmitters to produce spastic paralysis, clostridial neurotoxins are described as the most toxic substances known | ? | - |
? | |
synaptobrevin + H2O | Clostridium tetani Harvard | enzyme disables neuroexocytosis apparatus, acts at spinal inhibitory interneurons, blocking release of various neurotransmitters to produce spastic paralysis, clostridial neurotoxins are described as the most toxic substances known | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetani | - |
all toxigenic strains synthesize only one type of neurotoxin | - |
Clostridium tetani | - |
Harvard | - |
Clostridium tetani Harvard | - |
Harvard | - |
Oxidation Stability | Organism |
---|---|
extremely sensitive to oxidants | Clostridium tetani |
Purification (Comment) | Organism |
---|---|
single-chain, two-chain and L-chain form | Clostridium tetani |
very toxic! Booster injection of tetanus toxoid before starting research with tetanus toxin advisable, human anti-tetanus neurotoxin antibodies available | Clostridium tetani |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Hydrolysis of -Gln76-/-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP) | structure and mechanism | Clostridium tetani |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Clostridium tetani | - |
Storage Stability | Organism |
---|---|
-80°C, in 10 mM HEPES buffer, pH 7.2, 50 mM NaCl, after freezing in liquid N2, stable | Clostridium tetani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | synaptobrevin-1 (with Val76 instead of Gln76) or short peptides containing the cleavage site of the target protein | Clostridium tetani | ? | - |
? | |
additional information | synaptobrevin-1 (with Val76 instead of Gln76) or short peptides containing the cleavage site of the target protein | Clostridium tetani Harvard | ? | - |
? | |
Synaptobrevin + H2O | i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B | Clostridium tetani | Hydrolyzed synaptobrevin | - |
? | |
Synaptobrevin + H2O | i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B | Clostridium tetani Harvard | Hydrolyzed synaptobrevin | - |
? | |
synaptobrevin + H2O | enzyme disables neuroexocytosis apparatus, acts at spinal inhibitory interneurons, blocking release of various neurotransmitters to produce spastic paralysis, clostridial neurotoxins are described as the most toxic substances known | Clostridium tetani | ? | - |
? | |
synaptobrevin + H2O | enzyme disables neuroexocytosis apparatus, acts at spinal inhibitory interneurons, blocking release of various neurotransmitters to produce spastic paralysis, clostridial neurotoxins are described as the most toxic substances known | Clostridium tetani Harvard | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium tetani |