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Literature summary for 3.4.24.65 extracted from

  • Fulcher, Y.G.; Van Doren, S.R.
    Remote exosites of the catalytic domain of matrix metalloproteinase-12 enhance elastin degradation (2011), Biochemistry, 50, 9488-9499.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.65

Protein Variants

Protein Variants Comment Organism
A164V mutation inconsequential to elastolysis Homo sapiens
A182G mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows strong decreased (kcat/Km) toward fEln-100 compared to wild-type Homo sapiens
D124Q mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows decreased (kcat/Km) toward fEln-100 compared to wild-type Homo sapiens
D124Q/A182G combination of two well-separated mutations further reduces activity toward both fEln-100 and elastin-fluorescein compared to the single mutations, mutant shows decreased activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 Homo sapiens
D124Q/I180S combination of two well-separated mutations further reduces activity toward both fEln-100 and elastin-fluorescein compared to the single mutations, mutant shows decreased activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 Homo sapiens
D124Q/M156E combination of two well-separated mutations further reduces activity toward both fEln-100 and elastin-fluorescein compared to the single mutations, mutant retains wild-type activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 Homo sapiens
D124Q/M156E/A182G mutant confers a significantly greater loss of catalytic efficiency in digesting fEln-100 than the parental double mutant D124Q/M156E, catalytic activity toward MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is highly decreased compared to wild-type, compared to triple mutant D124Q/M156E/F185Y and D124Q/M156E/T205K catalytic efficacy toward fEln-100 is highly decreased Homo sapiens
D124Q/M156E/F185Y mutant confers a significantly greater loss of catalytic efficiency in digesting fEln-100 than the parental double mutant D124Q/M156E, catalytic activity toward MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is not affected compared to wild-type, compared to triple mutant D124Q/M156E/I180S and D124Q/M156E/A182G catalytic efficacy toward fEln-100 is moderately decreased Homo sapiens
D124Q/M156E/I180S mutant confers a significantly greater loss of catalytic efficiency in digesting fEln-100 than the parental double mutant D124Q/M156E, catalytic activity toward MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is highly decreased compared to wild-type, compared to triple mutant D124Q/M156E/F185Y and D124Q/M156E/T205K catalytic efficacy toward fEln-100 is highly decreased Homo sapiens
D124Q/M156E/T205K mutant confers a significantly greater loss of catalytic efficiency in digesting fEln-100 than the parental double mutant D124Q/M156E, catalytic activity toward MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 is not affected compared to wild-type, compared to triple mutant D124Q/M156E/I180S and D124Q/M156E/A182G catalytic efficacy toward fEln-100 is moderately decreased Homo sapiens
D200E mutation inconsequential to elastolysis Homo sapiens
G166R mutation inconsequential to elastolysis Homo sapiens
I180S mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows strong decreased (kcat/Km) toward fEln-100 compared to wild-type Homo sapiens
I255V mutation inconsequential to elastolysis Homo sapiens
K148T mutation inconsequential to elastolysis Homo sapiens
M103F mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows decreased catalytic efficacy (kcat/Km) toward fEln-100 compared to wild-type Homo sapiens
M156E mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows strong decreased (kcat/Km) toward fEln-100 compared to wild-type Homo sapiens
M156E/A182G kcat (fEln-100) is twice that of wild-type, mutant retains wild-type activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 Homo sapiens
M156E/I180S combination of two well-separated mutations further reduces activity toward both fEln-100 and elastin-fluorescein compared to the single mutations, mutant shows decreased activity towards MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 Homo sapiens
N153Y mutation inconsequential to elastolysis Homo sapiens
R117S mutant retains similar activity toward substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Km and kcat similar to wild-type, kcat/Km), mutant shows decreased (kcat/Km) toward fEln-100 compared to wild-type Homo sapiens
S142E mutation inconsequential to elastolysis Homo sapiens
V144A mutation inconsequential to elastolysis Homo sapiens
V162S mutation inconsequential to elastolysis Homo sapiens
Y132A mutation inconsequential to elastolysis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 fEln-100 pH 7.5, 25°C, wild-type Homo sapiens
0.00109
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/A182G Homo sapiens
0.00114
-
 fEln-100 pH 7.5, 25°C, mutant A182G Homo sapiens
0.00115
-
 fEln-100 pH 7.5, 25°C, mutant M103F Homo sapiens
0.00115
-
 fEln-100 pH 7.5, 25°C, mutant R117S Homo sapiens
0.00119
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/I180S Homo sapiens
0.00127
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/I180S Homo sapiens
0.0013
-
 fEln-100 pH 7.5, 25°C, mutant I180S Homo sapiens
0.00135
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/F185Y Homo sapiens
0.00145
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/T205K Homo sapiens
0.00171
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/A182G Homo sapiens
0.00175
-
 fEln-100 pH 7.5, 25°C, mutant D124Q Homo sapiens
0.00239
-
 fEln-100 pH 7.5, 25°C, mutant M156E Homo sapiens
0.0034
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E Homo sapiens
0.00399
-
 fEln-100 pH 7.5, 25°C, mutant M156E/I180S Homo sapiens
0.00466
-
 fEln-100 pH 7.5, 25°C, mutant M156E/A182G Homo sapiens
0.113
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/T205K Homo sapiens
0.113
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant R117S Homo sapiens
0.118
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E/A182G Homo sapiens
0.119
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E/I180S Homo sapiens
0.124
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E Homo sapiens
0.125
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q Homo sapiens
0.125
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E Homo sapiens
0.128
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M103F Homo sapiens
0.129
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, wild-type Homo sapiens
0.13
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant A182G Homo sapiens
0.135
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/F185Y Homo sapiens
0.17
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant I180S Homo sapiens
0.18
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/I180S Homo sapiens
0.194
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/A182G Homo sapiens
0.202
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/A182G Homo sapiens
0.241
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/I180S Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fEln-100 + H2O alpha-elastin Homo sapiens ?
-
 ?
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 + H2O soluble Knight's peptide substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MMP-12
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/A182G Homo sapiens
0.001
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/I180S Homo sapiens
0.0018
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/A182G Homo sapiens
0.0024
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/I180S Homo sapiens
0.0037
-
 fEln-100 pH 7.5, 25°C, mutant I180S Homo sapiens
0.0048
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/F185Y Homo sapiens
0.0052
-
 fEln-100 pH 7.5, 25°C, mutant M156E/I180S Homo sapiens
0.0057
-
 fEln-100 pH 7.5, 25°C, mutant A182G Homo sapiens
0.0065
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/T205K Homo sapiens
0.0072
-
 fEln-100 pH 7.5, 25°C, mutant R117S Homo sapiens
0.0092
-
 fEln-100 pH 7.5, 25°C, mutant M103F Homo sapiens
0.01
-
 fEln-100 pH 7.5, 25°C, wild-type Homo sapiens
0.0117
-
 fEln-100 pH 7.5, 25°C, mutant M156E Homo sapiens
0.0124
-
 fEln-100 pH 7.5, 25°C, mutant D124Q Homo sapiens
0.0143
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E Homo sapiens
0.021
-
 fEln-100 pH 7.5, 25°C, mutant M156E/A182G Homo sapiens
6.8
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E/I180S Homo sapiens
8.4
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/A182G Homo sapiens
9.7
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/I180S Homo sapiens
10.6
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/A182G Homo sapiens
13.7
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant R117S Homo sapiens
15.6
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant A182G Homo sapiens
16.2
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/I180S Homo sapiens
16.7
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E Homo sapiens
16.9
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E Homo sapiens
17.1
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q Homo sapiens
17.2
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E/A182G Homo sapiens
17.3
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, wild-type Homo sapiens
18
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/F185Y Homo sapiens
18
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/T205K Homo sapiens
18.7
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M103F Homo sapiens
24.1
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant I180S Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.59
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/A182G Homo sapiens
0.82
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/I180S Homo sapiens
1.31
-
 fEln-100 pH 7.5, 25°C, mutant M156E/I180S Homo sapiens
1.66
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/A182G Homo sapiens
1.855
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/I180S Homo sapiens
2.86
-
 fEln-100 pH 7.5, 25°C, mutant I180S Homo sapiens
3.57
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/F185Y Homo sapiens
4.21
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E Homo sapiens
4.5
-
 fEln-100 pH 7.5, 25°C, mutant D124Q/M156E/T205K Homo sapiens
4.65
-
 fEln-100 pH 7.5, 25°C, mutant M156E/A182G Homo sapiens
4.89
-
 fEln-100 pH 7.5, 25°C, mutant M156E Homo sapiens
4.97
-
 fEln-100 pH 7.5, 25°C, mutant A182G Homo sapiens
7.06
-
 fEln-100 pH 7.5, 25°C, mutant D124Q Homo sapiens
7.13
-
 fEln-100 pH 7.5, 25°C, mutant R117S Homo sapiens
8.04
-
 fEln-100 pH 7.5, 25°C, mutant M103F Homo sapiens
10.69
-
 fEln-100 pH 7.5, 25°C, wild-type Homo sapiens
51.57
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/A182G Homo sapiens
53.66
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/I180S Homo sapiens
54.61
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/A182G Homo sapiens
57.42
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E/I180S Homo sapiens
67.23
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/I180S Homo sapiens
120.1
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant A182G Homo sapiens
120.8
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant R117S Homo sapiens
133.4
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/F185Y Homo sapiens
133.8
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, wild-type Homo sapiens
134.7
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E Homo sapiens
135.2
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E Homo sapiens
136.7
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q Homo sapiens
141.8
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant I180S Homo sapiens
145.4
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M156E/A182G Homo sapiens
146.3
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant M103F Homo sapiens
159.7
-
 Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 pH 7.5, 25°C, mutant D124Q/M156E/T205K Homo sapiens