Any feedback?
Literature summary for 3.4.24.65 extracted from
- Cloning and characterization of a unique elastolytic metalloproteinase produced by human alveolar macrophages (1993), J. Biol. Chem., 268, 23824-23829.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| Purification in the presence of EDTA prevents activation of proenzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha2-Macroglobulin | SDS abolishes inhibition | Homo sapiens | |
| tissue inhibitor of metalloproteinase | i.e. TIMP-1, recombinant enzyme | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | recombinant enzyme from Escherichia coli | Homo sapiens | 5829 | - |
| extracellular | native enzyme | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | metalloendopeptidase, contains zinc-binding motif, the catalytic domain that coordiantes the active-site zinc | Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the open-reading frames predict proenzymes of MW 54000 | Homo sapiens |
| 22000 | - |
x * 22000, human, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, recombinant or native enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| macrophage | alveolar macrophages | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Elastin + H2O | - |
Homo sapiens | More | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22000, human, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Human macrophage metalloelastase | HME | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
