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Literature summary for 3.4.24.65 extracted from

  • Banda, M.J.; Werb, Z.
    Mouse macrophage elastase. Purification and characterization as a metalloproteinase (1981), Biochem. J., 193, 589-605.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.65

General Stability

General Stability Organism
Buffers with high concentration of Ca2+ stabilize during purification Mus musculus
Dialysis against 25 mM Tris-HCl, pH 7.6 inactivates, Ca2+ restores activity Mus musculus
Purified enzyme, not partially purified preparation, unstable in either glycerol or sucrose velocity gradients Mus musculus
Stable to freeze-thawing Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
alpha2-Macroglobulin SDS abolishes inhibition Mus musculus
dithiothreitol
-
 Mus musculus
EDTA Zn2+ at a 1:10 ratio of Zn2+/EDTA protects and reverses, not Ca2+, no reversal by Co2+ or Mg2+ Mus musculus
EGTA less effective than EDTA, Ca2+ at a 3:1 ratio of Ca2+/EDTA protects and reverses partially, Zn2+ at a 1:10 ratio of Zn2+/EGTA protects and reverses Mus musculus
additional information diisopropylphosphorofluoridate, PCMB, NEM, mersalyl, soybean trypsin inhibitor, acetyltetraalanylchloromethane, phosphoramidon, glycoprotein tissue inhibitor, alpha1-proteinase inhibitor; no inhibition by PMSF (form B) Mus musculus
organic solvents enzyme form B, above 0.5% v/v Mus musculus
Rabbit antiserum against purified elastase form B
-
 Mus musculus
Zn2+ at a 3fold molar excess of Zn2+ over chelating agent Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Mus musculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 mouse, form B and C, gel filtration, sucrose density gradient centrifugation Mus musculus
57000
-
 mouse, form A, sucrose density gradient centrifugation, gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3 chromatographically distinct forms A, B and C, predominant form is B Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
macrophage tissue culture medium of colchicin-treated inflammatory peritoneal macrophages Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus

Storage Stability

Storage Stability Organism
-80°C, in 50 mM Tris-HCl, pH 7.6, 150 mM NaCl, 50 mM CaCl2, at least 3 months Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Elastin + H2O preferentially cleaves on amino-side of Leus, not Val Mus musculus More products of elastin hydrolysis are predominantly high MW peptides, MW 90000-100000 ?
additional information acetyl-Ala-Ala-Ala 4-nitroanilide or benzyloxycarbonyl-Ala 2-naphthyl esters Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 22000, mouse, form B and C, SDS-PAGE Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 enzyme form B Mus musculus