Literature summary for 3.4.24.64 extracted from

Taylor, A.B.; Smith, B.S.; Kitada, S.; Kojima, K.; Miyaura, H.; Otwinowski, Z.; Ito, A.; Deisenhofer, J.
Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences (2001), Structure, 9, 615-625.

Search for more literature for 3.4.24.64

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant enzyme overexpressed in Escherichia coli BL21(DE3)	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant MPP complexed with 2 different synthtic peptide substrates, crystallized by hanging-drop vapor diffusion method, unit cell constants a = 133 A, b = 178 A, c = 201 A, space group P2(1)2(1)2(1)	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
E177G	mutation does not affect enzymatic activity	Saccharomyces cerevisiae
E217G	mutation does not affect enzymatic activity	Saccharomyces cerevisiae
E73Q	cleavage-deficient mutant	Saccharomyces cerevisiae
Q350R	mutation does not affect enzymatic activity	Saccharomyces cerevisiae
S20A	mutation does not affect enzymatic activity	Saccharomyces cerevisiae
S84P	mutation does not affect enzymatic activity	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	localized in the active site cavity	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
100000	-	-	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	yeast	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and mutant enzyme	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
COX IV 2-25 + H2O	-	Saccharomyces cerevisiae	?	-	?
MDH 2-17 + H2O	-	Saccharomyces cerevisiae	?	-	?

Subunits

Subunits	Comment	Organism
heterodimer	-	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
MPP	-	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)