Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Saccharomyces cerevisiae | |
additional information | prepeptides pSynB2, p15 | Saccharomyces cerevisiae | |
Prepeptide p25 | derived from amino terminal of cytochrome oxidase subunit IV precursor, kinetics | Saccharomyces cerevisiae | |
Prepeptide p34 | derived from amino terminal of cytochrome oxidase subunit IV precursor, kinetics | Saccharomyces cerevisiae | |
Prepeptide pSynC or pSynA2 | less effective than p25 or p34 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | matrix | Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | metalloprotease | Saccharomyces cerevisiae | |
Co2+ | requirement (in vitro, in vivo not identified yet) | Saccharomyces cerevisiae | |
Mn2+ | requirement | Saccharomyces cerevisiae | |
Mn2+ | metalloprotease | Saccharomyces cerevisiae | |
Mn2+ | requirement (in vitro, in vivo not identified yet) | Saccharomyces cerevisiae | |
additional information | atomic absorption spectroscopy: purified MAS1/MAS2-holoenzyme lacks significant amounts of zinc, manganese or cobalt, probably lost during purification | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence comparison on the basis of statistical analysis, FASTP program | Saccharomyces cerevisiae |
48800 | - |
1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence | Saccharomyces cerevisiae |
51800 | - |
1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence | Saccharomyces cerevisiae |
100000 | - |
sedimentation velocity analysis | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
overproducing strain VGA | - |
Purification (Comment) | Organism |
---|---|
2 components: MAS1 and MAS2 | Saccharomyces cerevisiae |
if MAS1 is overproduced in the absence of MAS2 it is insoluble and not suitable for purification, it is therefore purified from the purified holoenzyme | Saccharomyces cerevisiae |
improved procedure | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 | mechanism | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000001 | - |
artificial fusion protein | Saccharomyces cerevisiae |
0.000003 | - |
P25-peptide | Saccharomyces cerevisiae |
1.785 | - |
strain JKR 102(YEp13-MAS1), overexpressing MAS1 | Saccharomyces cerevisiae |
2.35 | - |
strain JKR 102 | Saccharomyces cerevisiae |
4.688 | - |
strain JKR 102(pCF35-MAS2), overexpressing MAS2 | Saccharomyces cerevisiae |
23.44 | - |
strain JKR 102(YEp13-MAS1 plus pCF35-MAS2), overexpressing MAS1 and MAS2 | Saccharomyces cerevisiae |
26.78 | - |
strain VGA | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate binds to MAS2, not MAS1 enzyme component (photocrosslinking experiment) | Saccharomyces cerevisiae | ? | - |
? | |
additional information | removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes | Saccharomyces cerevisiae | ? | - |
? | |
P-25 peptide + H2O | matrix-targeting peptide containing cleavage site of authentic precursor protein | Saccharomyces cerevisiae | ? | - |
? | |
Precytochrome b2-mouse dehydrofolate reductase fusion protein + H2O | - |
Saccharomyces cerevisiae | 31-aminoacid presequence + cytochrome b2-mouse dehydrofolate reductase protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence | Saccharomyces cerevisiae |