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Literature summary for 3.4.24.64 extracted from

  • Yang, M.; Geli, V.; Oppliger, W.; Suda, K.; James, P.; Schatz, G.
    The MAS-encoded processing protease of yeast mitochondria. Interaction of the purified enzyme with signal peptides and a purified precursor protein (1991), J. Biol. Chem., 266, 6416-6423.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Saccharomyces cerevisiae
additional information prepeptides pSynB2, p15 Saccharomyces cerevisiae
Prepeptide p25 derived from amino terminal of cytochrome oxidase subunit IV precursor, kinetics Saccharomyces cerevisiae
Prepeptide p34 derived from amino terminal of cytochrome oxidase subunit IV precursor, kinetics Saccharomyces cerevisiae
Prepeptide pSynC or pSynA2 less effective than p25 or p34 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion matrix Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ metalloprotease Saccharomyces cerevisiae
Co2+ requirement (in vitro, in vivo not identified yet) Saccharomyces cerevisiae
Mn2+ requirement Saccharomyces cerevisiae
Mn2+ metalloprotease Saccharomyces cerevisiae
Mn2+ requirement (in vitro, in vivo not identified yet) Saccharomyces cerevisiae
additional information atomic absorption spectroscopy: purified MAS1/MAS2-holoenzyme lacks significant amounts of zinc, manganese or cobalt, probably lost during purification Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid sequence comparison on the basis of statistical analysis, FASTP program Saccharomyces cerevisiae
48800
-
1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence Saccharomyces cerevisiae
51800
-
1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence Saccharomyces cerevisiae
100000
-
sedimentation velocity analysis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
overproducing strain VGA
-

Purification (Commentary)

Purification (Comment) Organism
2 components: MAS1 and MAS2 Saccharomyces cerevisiae
if MAS1 is overproduced in the absence of MAS2 it is insoluble and not suitable for purification, it is therefore purified from the purified holoenzyme Saccharomyces cerevisiae
improved procedure Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 mechanism Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000001
-
artificial fusion protein Saccharomyces cerevisiae
0.000003
-
P25-peptide Saccharomyces cerevisiae
1.785
-
strain JKR 102(YEp13-MAS1), overexpressing MAS1 Saccharomyces cerevisiae
2.35
-
strain JKR 102 Saccharomyces cerevisiae
4.688
-
strain JKR 102(pCF35-MAS2), overexpressing MAS2 Saccharomyces cerevisiae
23.44
-
strain JKR 102(YEp13-MAS1 plus pCF35-MAS2), overexpressing MAS1 and MAS2 Saccharomyces cerevisiae
26.78
-
strain VGA Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate binds to MAS2, not MAS1 enzyme component (photocrosslinking experiment) Saccharomyces cerevisiae ?
-
?
additional information removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes Saccharomyces cerevisiae ?
-
?
P-25 peptide + H2O matrix-targeting peptide containing cleavage site of authentic precursor protein Saccharomyces cerevisiae ?
-
?
Precytochrome b2-mouse dehydrofolate reductase fusion protein + H2O
-
Saccharomyces cerevisiae 31-aminoacid presequence + cytochrome b2-mouse dehydrofolate reductase protein
-
?

Subunits

Subunits Comment Organism
dimer 1 * 48800 (MAS1) + 1 * 51800 (MAS2), Saccharomyces cerevisiae, calculated from amino acid sequence Saccharomyces cerevisiae