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Literature summary for 3.4.24.64 extracted from

  • Yang, M.; Jensen, R.E.; Yaffe, M.P.; Oppliger, W.; Schatz, G.
    Import of proteins into yeast mitochondria: the purified matrix processing protease contains two subunits which are encoded by the nuclear MAS1 and MAS2 genes (1988), EMBO J., 7, 3857-3862.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.64

General Stability

General Stability Organism
Separation of the loosely associated subunits of MW 48000 and 51000 causes complete loss of activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Saccharomyces cerevisiae
additional information PMSF Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion matrix Saccharomyces cerevisiae 5739
-
soluble
-
 Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE Saccharomyces cerevisiae
50000
-
 gel filtration Saccharomyces cerevisiae
51000
-
 1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE Saccharomyces cerevisiae
100000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes ?
-
 ?
additional information Saccharomyces cerevisiae D-273-10B removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae D-273-10B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from soluble matrix fraction of isolated mitochondria Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 cleaves amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins Saccharomyces cerevisiae
a

Storage Stability

Storage Stability Organism
-70°C, after quick-freezing in liquid nitrogen, at least 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Cytochrome b2 precursor + H2O
-
 Saccharomyces cerevisiae Cytochrome b2 intermediate form
-
 ?
Cytochrome b2 precursor + H2O
-
 Saccharomyces cerevisiae D-273-10B Cytochrome b2 intermediate form
-
 ?
Cytochrome c oxidase subunit IV precursor + H2O
-
 Saccharomyces cerevisiae Cytochrome c oxidase subunit IV
-
 ?
Cytochrome c oxidase subunit IV precursor + H2O
-
 Saccharomyces cerevisiae D-273-10B Cytochrome c oxidase subunit IV
-
 ?
Cytochrome c1 precursor + H2O membrane-bound enzyme Saccharomyces cerevisiae Cytochrome c1 intermediate form
-
 ?
F1-ATPase beta-subunit precursor + H2O
-
 Saccharomyces cerevisiae F1-ATPase beta-subunit
-
 ?
F1-ATPase beta-subunit precursor + H2O
-
 Saccharomyces cerevisiae D-273-10B F1-ATPase beta-subunit
-
 ?
Mitochondrial alcohol dehydrogenase precursor + H2O
-
 Saccharomyces cerevisiae Mitochondrial alcohol dehydrogenase
-
 ?
additional information the large subunit alone has no cleavage activity Saccharomyces cerevisiae ?
-
 ?
additional information removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes Saccharomyces cerevisiae ?
-
 ?
additional information the large subunit alone has no cleavage activity Saccharomyces cerevisiae D-273-10B ?
-
 ?
additional information removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes Saccharomyces cerevisiae D-273-10B ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE Saccharomyces cerevisiae
More Saccharomyces cerevisiae has two loosely associated non-identical subunits that dissociate upon gel filtration Saccharomyces cerevisiae
More the large subunit alone has no cleavage activity Saccharomyces cerevisiae