Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | excess Zn2+ or Co2+ partially restores; in vitro and in vivo | Saccharomyces cerevisiae | |
EDTA | excess Zn2+, Co2+ partially restores | Saccharomyces cerevisiae | |
GTP | Zn2+ or Co2+ in excess partially restores | Saccharomyces cerevisiae | |
additional information | 4-aminobenzamidine; diisopropyl fluoride; isolated from bovine; no inhibition by leupeptin or pepstatin; no inhibition by serine protease inhibitors, e.g. antipain, chymostatin; no inhibition by tryptic inhibitors; pancreas, chicken egg white or soybean; PMSF | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | half-maximal processing of in vitro synthesized precursors to their respective mature forms: F1-ATPase beta-subunit at 1.5 min, cytochrome b2 at 22 min and cytochrome c oxidase at 4 min | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | matrix | Saccharomyces cerevisiae | 5739 | - |
soluble | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | metalloprotease | Saccharomyces cerevisiae | |
Co2+ | activation of chelator-inactivated enzyme | Saccharomyces cerevisiae | |
Zn2+ | activation of chelator-inactivated enzyme | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | - |
mutant strains GL-1 (heme-deficient) or D273-10B-1 (rho-) | - |
Saccharomyces cerevisiae D-273-10B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 | limited proteolysis: cleaves precursors of mitochondrial proteins to their mature form, not further | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Citrate synthase precursor + H2O | - |
Saccharomyces cerevisiae | Citrate synthase | - |
? | |
Cytochrome b2 precursor + H2O | - |
Saccharomyces cerevisiae | Cytochrome b2 intermediate form | - |
? | |
Cytochrome c oxidase subunit V precursor + H2O | - |
Saccharomyces cerevisiae | Cytochrome c oxidase subunit V | - |
? | |
Cytochrome c1 precursor + H2O | membrane-bound enzyme | Saccharomyces cerevisiae | Cytochrome c1 intermediate form | - |
? | |
F1-ATPase alpha-subunit precursor + H2O | - |
Saccharomyces cerevisiae | F1-ATPase alpha-subunit | - |
? | |
F1-ATPase beta-subunit precursor + H2O | - |
Saccharomyces cerevisiae | F1-ATPase beta-subunit | - |
? | |
additional information | highly specific enzyme | Saccharomyces cerevisiae | ? | - |
? | |
additional information | No substrates are several non-mitochondrial proteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | e.g. bovine serum albumin, mouse immunoglobulin G, yeast hexokinase or yeast tryptophan synthase, neither in native nor in heat or pH-denatured form | Saccharomyces cerevisiae | ? | - |
? | |
additional information | No substrates are mature mitochondrial proteins | Saccharomyces cerevisiae | ? | - |
? | |
additional information | No substrates are denatured enzyme precursors | Saccharomyces cerevisiae | ? | - |
? | |
additional information | highly specific enzyme | Saccharomyces cerevisiae D-273-10B | ? | - |
? | |
additional information | No substrates are several non-mitochondrial proteins | Saccharomyces cerevisiae D-273-10B | ? | - |
? | |
additional information | e.g. bovine serum albumin, mouse immunoglobulin G, yeast hexokinase or yeast tryptophan synthase, neither in native nor in heat or pH-denatured form | Saccharomyces cerevisiae D-273-10B | ? | - |
? | |
additional information | No substrates are mature mitochondrial proteins | Saccharomyces cerevisiae D-273-10B | ? | - |
? | |
additional information | No substrates are denatured enzyme precursors | Saccharomyces cerevisiae D-273-10B | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharomyces cerevisiae |