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Literature summary for 3.4.24.64 extracted from

  • Böhni, P.C.; Daum, G.; Schatz, G.
    Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides (1983), J. Biol. Chem., 258, 4937-4943.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline excess Zn2+ or Co2+ partially restores; in vitro and in vivo Saccharomyces cerevisiae
EDTA excess Zn2+, Co2+ partially restores Saccharomyces cerevisiae
GTP Zn2+ or Co2+ in excess partially restores Saccharomyces cerevisiae
additional information 4-aminobenzamidine; diisopropyl fluoride; isolated from bovine; no inhibition by leupeptin or pepstatin; no inhibition by serine protease inhibitors, e.g. antipain, chymostatin; no inhibition by tryptic inhibitors; pancreas, chicken egg white or soybean; PMSF Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information half-maximal processing of in vitro synthesized precursors to their respective mature forms: F1-ATPase beta-subunit at 1.5 min, cytochrome b2 at 22 min and cytochrome c oxidase at 4 min Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion matrix Saccharomyces cerevisiae 5739
-
soluble
-
Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ metalloprotease Saccharomyces cerevisiae
Co2+ activation of chelator-inactivated enzyme Saccharomyces cerevisiae
Zn2+ activation of chelator-inactivated enzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae
-
mutant strains GL-1 (heme-deficient) or D273-10B-1 (rho-)
-
Saccharomyces cerevisiae D-273-10B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 limited proteolysis: cleaves precursors of mitochondrial proteins to their mature form, not further Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Citrate synthase precursor + H2O
-
Saccharomyces cerevisiae Citrate synthase
-
?
Cytochrome b2 precursor + H2O
-
Saccharomyces cerevisiae Cytochrome b2 intermediate form
-
?
Cytochrome c oxidase subunit V precursor + H2O
-
Saccharomyces cerevisiae Cytochrome c oxidase subunit V
-
?
Cytochrome c1 precursor + H2O membrane-bound enzyme Saccharomyces cerevisiae Cytochrome c1 intermediate form
-
?
F1-ATPase alpha-subunit precursor + H2O
-
Saccharomyces cerevisiae F1-ATPase alpha-subunit
-
?
F1-ATPase beta-subunit precursor + H2O
-
Saccharomyces cerevisiae F1-ATPase beta-subunit
-
?
additional information highly specific enzyme Saccharomyces cerevisiae ?
-
?
additional information No substrates are several non-mitochondrial proteins Saccharomyces cerevisiae ?
-
?
additional information e.g. bovine serum albumin, mouse immunoglobulin G, yeast hexokinase or yeast tryptophan synthase, neither in native nor in heat or pH-denatured form Saccharomyces cerevisiae ?
-
?
additional information No substrates are mature mitochondrial proteins Saccharomyces cerevisiae ?
-
?
additional information No substrates are denatured enzyme precursors Saccharomyces cerevisiae ?
-
?
additional information highly specific enzyme Saccharomyces cerevisiae D-273-10B ?
-
?
additional information No substrates are several non-mitochondrial proteins Saccharomyces cerevisiae D-273-10B ?
-
?
additional information e.g. bovine serum albumin, mouse immunoglobulin G, yeast hexokinase or yeast tryptophan synthase, neither in native nor in heat or pH-denatured form Saccharomyces cerevisiae D-273-10B ?
-
?
additional information No substrates are mature mitochondrial proteins Saccharomyces cerevisiae D-273-10B ?
-
?
additional information No substrates are denatured enzyme precursors Saccharomyces cerevisiae D-273-10B ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Saccharomyces cerevisiae