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Literature summary for 3.4.24.63 extracted from
- Expression of meprin subunit precursors. Membrane anchoring through the beta subunit and mechanism of zymogen activation (1994), J. Biol. Chem., 269, 7682-7688.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Trypsin | peptidase activity of expressed meprin B precursor is absolutely dependent on activation by trypsin | Rattus norvegicus | |
| Trypsin | activation is accompanied by reduction in size of 8 kDa | Rattus norvegicus | |
| Trypsin | by limited proteolysis | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| rat, expressed in human kidney cell line 293, the expressed protein displays no detectable peptidase activity unless it is activated by removal of amino-terminal prosequence by limited trypsin digestion | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | microvillar membrane, integral membrane protein | Rattus norvegicus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
x * 74000, deglycosylated enzyme, SDS-PAGE under reducing conditions | Rattus norvegicus |
| 84000 | - |
x * 84000, expressed in human kidney 293 cells, deglycosylated form, SDS-PAGE under reducing conditions | Rattus norvegicus |
| 97000 | - |
x * 97000, expressed in human kidney 293 cells, SDS-PAGE under reducing conditions | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-glycosidase F-sensitive | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed in human kidney cell line 293, papain-solubilized | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | poor substrate | Rattus norvegicus | ? | - |
? |  |
| additional information | no substrates are glutaryl-Ala-Ala-Phe 4-methoxynaphthalamine 2-amide | Rattus norvegicus | ? | - |
? | |
| Succinyl-Ala-Ala-Ala 4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Succinyl-Ala-Ala-Val-Ala 4-nitroanilide + H2O | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 74000, deglycosylated enzyme, SDS-PAGE under reducing conditions | Rattus norvegicus |
| oligomer | x * 84000, expressed in human kidney 293 cells, deglycosylated form, SDS-PAGE under reducing conditions | Rattus norvegicus |
| oligomer | x * 97000, expressed in human kidney 293 cells, SDS-PAGE under reducing conditions | Rattus norvegicus |
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