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Literature summary for 3.4.24.63 extracted from

  • Kounnas, M.Z.; Woltz, R.L.; Gorbea, C.M.; Bond, J.S.
    Meprin-A and -B. Cell surface endopeptidases of the mouse kidney (1991), J. Biol. Chem., 266, 17350-17357.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Trypsin activation, 5-20fold Mus musculus
Trypsin only with larger substrates (azocasein or insulin B), not smaller substrates Mus musculus
Trypsin by limited proteolysis (2 h at 37°C, with tosyl-Phe chloromethyl ketone treated trypsin, in 20 mM Tris-HCl buffer, pH 7.5) Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline strong Mus musculus
cysteine
-
Mus musculus
EDTA strong Mus musculus
additional information compound E-64; phosphoramidon, iodoacetic acid, 3,4-dichloroisocoumarin or soybean trypsin inhibitor Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane renal brush-border membrane Mus musculus 16020
-
additional information cell surface endopeptidase Mus musculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloendopeptidase according to inhibition profile Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition and secondary structure similar to meprin A Mus musculus
90000
-
x * 90000, mouse, SDS-PAGE under reducing or non-reducing conditions Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
inbred strain C3H/He
-
Mus musculus
-
male adults
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Mus musculus

Purification (Commentary)

Purification (Comment) Organism
papain-solubilized Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney brush border membrane Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
0.4763 mg azocasein/min mg protein Mus musculus
0.367 0.467 Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly as substrate Mus musculus
1.82
-
insulin B-chain as substrate Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O poor substrate Mus musculus ?
-
?
azocasein + H2O mouse enzyme: poor substrate unless activated by trypsin treatment (i.e. latent azocaseinase activity) Mus musculus ?
-
?
Insulin B-chain + H2O 4 cleavage sites (2 major and 2 minor): His5-Leu6, Leu6-Cys(SO3-)7, Ala14-Leu15, Cys(SO3-)19-Gly20 Mus musculus ?
-
?
Insulin B-chain + H2O yielding at least 7 product peptides Mus musculus ?
-
?
additional information no substrates are nitrobradykinin Mus musculus ?
-
?
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly + H2O synthetic octapeptide, derived from insulin B-chain Mus musculus ?
-
?

Subunits

Subunits Comment Organism
oligomer x * 90000, mouse, SDS-PAGE under reducing or non-reducing conditions Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
t1/2: 2.5 min, less stable than meprin A Mus musculus