Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | peptide I as substrate; strong, 2.5 mM | Bos taurus | |
EGTA | peptide I as substrate; strong, 2.5 mM | Bos taurus | |
additional information | [D-Lys11]-pro-oxytocin/neurophysin(1-20), Gly-Gly-Lys-D-Arg-Ala-Val, Cys-Pro-Leu-Gly-Gly-Lys-D-Arg-Ala-Val; no inhibition by PMSF, pepstatin | Bos taurus | |
p-chloromercuriphenylsulfonic acid | 2.5 mM; peptide I as substrate | Bos taurus | |
PCMB | 2.5 mM; peptide I as substrate | Bos taurus | |
[D-Arg12]-Pro-oxytocin/neurophysin(1-20) | kinetics; peptide I as substrate | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
peptide I | neurohypophyseal enzyme | Bos taurus | |
0.2 | - |
peptide I | corpus luteum | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
secretory granule | - |
Bos taurus | 30141 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
bovine, PAGE, 2 isoforms, MW 52000 and 58000 | Bos taurus |
58000 | - |
bovine, PAGE, 2 isoforms, MW 52000 and 58000 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
cow | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Bos taurus |
from purified vesicles | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
corpus luteum | - |
Bos taurus | - |
hypophysis | pituitary neurosecretory granule | Bos taurus | - |
neurohypophysis | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide I + H2O | - |
Bos taurus | ? | - |
? | |
Pro-oxytocin/neurophysin(1-20) + H2O | i.e. model substrate peptide I | Bos taurus | Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20) | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE, 1 * 58000, SDS-PAGE, 2 isoforms, MW 52000 and 58000 | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.9 or 6.9 (corpus luteum, different enzyme preparations) | Bos taurus |
additional information | - |
pI: 6.9 and 7.1 (2 isoforms) | Bos taurus |
7 | - |
around, peptide I as substrate | Bos taurus |