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Literature summary for 3.4.24.59 extracted from
- Mitochondrial intermediate peptidase: Expression in Escherichia coli and improvement of its enzymatic activity detection with FRET substrates (2010), Biochem. Biophys. Res. Commun., 391, 123-128.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | activates enzymatic activity up to 0.1 mM | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | total inhibition at 10 mM | Homo sapiens | |
| bestatin | significant inhibition | Homo sapiens | |
| captopril | - |
Homo sapiens | |
| DTT | inhibits enzymatic activity above to 0.1 mM | Homo sapiens | |
| EDTA | 90% inhibitiond by EDTA (10 mM) | Homo sapiens | |
| JA-2 | - |
Homo sapiens | |
| thiorphan | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
Abz-GFSPFRQ-EDDnp | pH 7.4, 37°C, 0.1 mM DTT | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78640 | - |
mass spectroscopy | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography using Ni-Sepharose resin and ion exchange chromatography with a Hi-trap resource Q column | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Abz-GFSPFRQ-EDDnp + H2O | - |
Homo sapiens | Abz-GF + SPFRQ-EDDnp | - |
? | |
| Abz-RPPGFSPFRQ-EDDnp + H2O | - |
Homo sapiens | Abz-RPPGF + SPFRQ-EDDnp | - |
? | |
| bradykinin + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hMIP | - |
Homo sapiens |
| mitochondrial intermediate peptidase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
thiorphan | pH 7.4, 37°C | Homo sapiens | |
| 0.007 | - |
JA-2 | pH 7.4, 37°C | Homo sapiens | |
| 0.057 | - |
captopril | pH 7.4, 37°C | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 40 | - |
Abz-GFSPFRQ-EDDnp | pH 7.4, 37°C, 0.1 mM DTT | Homo sapiens | |
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