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Literature summary for 3.4.24.58 extracted from
- Allosteric aptamers controlling a signal amplification cascade allow visual detection of molecules at picomolar concentrations (2006), Biochemistry, 45, 2461-2466.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| RNA132 | an allosteric RNA aptamer, secondary structure folding, overview, causes 87% inhibition of the enzyme in the RVV-X-SPZXa assay, using a chromogenic substrate for the activated factor X, releasing the chromophore, 4-nitroanilide acetate, the snake venom protease competes with the human or murine vascular endothelial growth factor, VEGF165, for binding to RNA132 and reverses the inhibitory activity of RNA132 on RVV-X and restores its enzymatic activity, the VEGF165 of zebrafish functions partially, mapping of binding sites, overview | Daboia russelii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Daboia russelii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Daboia russelii |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Daboia russelii | the enzyme triggers the blood coagulation cascade, which results in an eye-visible phase transition, i.e. precipitation, of polystyrene microspheres bound to clotted fibrin, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daboia russelii | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Daboia russelii | - |
| venom | - |
Daboia russelii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
determination of enzyme activity in the RVV-X-SPZXa assay, using a chromogenic substrate for the activated factor X, releasing the chromophore, 4-nitroanilide acetate | Daboia russelii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor X + H2O | - |
Daboia russelii | factor Xa + propeptide of factor Xa | - |
? | |
| additional information | the enzyme triggers the blood coagulation cascade, which results in an eye-visible phase transition, i.e. precipitation, of polystyrene microspheres bound to clotted fibrin, overview | Daboia russelii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Russells viper venom factor X activator | - |
Daboia russelii |
| RVV-X | - |
Daboia russelii |
| snake venom protease | - |
Daboia russelii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Daboia russelii |
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Release 2023.1 (January 2023)
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