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Literature summary for 3.4.24.56 extracted from

  • Song, E.S.; Daily, A.; Fried, M.G.; Juliano, M.A.; Juliano, L.; Hersh, L.B.
    Mutation of active site residues of insulin-degrading enzyme alters allosteric interactions (2005), J. Biol. Chem., 280, 17701-17706.
    View publication on PubMed
Search for more literature for 3.4.24.56

Protein Variants

Protein Variants Comment Organism
E111A change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is dampened. Mutant recognizes additional cleavage sites in substrate beta-endorphin Rattus norvegicus
E111F change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is dampened. Mutant recognizes additional cleavage sites in substrate beta-endorphin Rattus norvegicus
E111L change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is dampened. Mutant recognizes additional cleavage sites in substrate beta-endorphin Rattus norvegicus
E111V change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is dampened. Mutant recognizes additional cleavage sites in substrate beta-endorphin Rattus norvegicus
H112D change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is changed to inhibition. Affinity to Zn2+ is lower than in wild-type Rattus norvegicus
H112Q change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is changed to inhibition. Affinity to Zn2+ is lower than in wild-type Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.1 mM, wild-type, 91% residual activity, mutants H112D, H112Q, less than 2.5% residual activity Rattus norvegicus
o-phenanthroline 0.1 mM, wild-type, 73% residual activity, mutants H112D, H112Q, less than 2.5% residual activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111L, pH 7.3 Rattus norvegicus
0.0156
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111V, pH 7.3 Rattus norvegicus
0.0274
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111F, pH 7.3 Rattus norvegicus
0.0287
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) wild-type, K0.5-value, pH 7.3 Rattus norvegicus
0.083
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant H112D, pH 7.3 Rattus norvegicus
0.158
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant H112Q, pH 7.3 Rattus norvegicus
0.204
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111A, pH 7.3 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus ?
-
 ?
amyloid beta-protein + H2O
-
 Rattus norvegicus ?
-
 ?
beta-endorphin + H2O
-
 Rattus norvegicus ?
-
 ?
insulin + H2O
-
 Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant H112D, pH 7.3 Rattus norvegicus
2.4
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111V, pH 7.3 Rattus norvegicus
6.5
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111L, pH 7.3 Rattus norvegicus
11.4
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111F, pH 7.3 Rattus norvegicus
17.5
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111A, pH 7.3 Rattus norvegicus
1953
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant H112Q, pH 7.3 Rattus norvegicus
162600
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) wild-type, pH 7.3 Rattus norvegicus