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Literature summary for 3.4.24.56 extracted from

  • Garcia, J.V.; Fenton, B.W.; Rosner, M.R.
    Isolation and characterization of an insulin-degrading enzyme from Drosophila melanogaster (1988), Biochemistry, 27, 4237-4244.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
bacitracin
-
Drosophila melanogaster
additional information not: aprotinin; not: pancreatic trypsin inhibitor Drosophila melanogaster
NEM
-
Drosophila melanogaster
PCMB
-
Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000003
-
Insulin procine substrate Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Drosophila melanogaster 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
Drosophila melanogaster, gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
insulin + H2O Drosophila melanogaster important role in the metabolism of insulin ?
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
Kc cells
-

Purification (Commentary)

Purification (Comment) Organism
-
Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
Kc cell
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Insulin + H2O not: individual A and B-chains of insulin Drosophila melanogaster Hydrolyzed insulin
-
?
Insulin + H2O equine Drosophila melanogaster Hydrolyzed insulin
-
?
insulin + H2O important role in the metabolism of insulin Drosophila melanogaster ?
-
?
Insulin growth factor II + H2O
-
Drosophila melanogaster ?
-
?
additional information insulin-like growth factor I Drosophila melanogaster ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 110000, Drosophila melanogaster, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Drosophila melanogaster

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 30% of activity maximum, pH 8.5: about 60% of activity maximum Drosophila melanogaster