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Literature summary for 3.4.24.55 extracted from
- Cloning, expression and characterization of a 46.5-kDa metallopeptidase from Bacillus halodurans H4 sharing properties with the pitrilysin family (2005), Biochim. Biophys. Acta, 1725, 136-143.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Halalkalibacterium halodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, 70% inhibition | Halalkalibacterium halodurans |  |
| 2-mercaptoethanol | 1% w/v, 95% inhibition | Halalkalibacterium halodurans | |
| Co2+ | 1 mM, complete inhibition | Halalkalibacterium halodurans | |
| Cu2+ | 1 mM, complete inhibition | Halalkalibacterium halodurans | |
| cysteine | 1 mM, complete inhibition | Halalkalibacterium halodurans | |
| E-64 | 1 mM, 46% inhibition | Halalkalibacterium halodurans | |
| EDTA | 1 mM, complete inhibition | Halalkalibacterium halodurans | |
| EGTA | 1 mM, complete inhibition | Halalkalibacterium halodurans | |
| Fe2+ | 1 mM, 76% inhibition | Halalkalibacterium halodurans | |
| N-ethylmaleimide | 1 mM, 95% inhibition | Halalkalibacterium halodurans | |
| Zn2+ | 1 mM, complete inhibition | Halalkalibacterium halodurans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Insulin B-chain | pH 8.0, 37°C | Halalkalibacterium halodurans | |
| 0.12 | - |
dynorphin A fragment 1-13 | pH 8.0, 37°C | Halalkalibacterium halodurans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | marked activation | Halalkalibacterium halodurans | |
| Zn2+ | protein contains 1 atom per molecule | Halalkalibacterium halodurans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46500 | - |
gel filtration | Halalkalibacterium halodurans |
| 46585 | - |
1 * 46500, SDS-PAGE, 1 * 46585, calculated | Halalkalibacterium halodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halalkalibacterium halodurans | - |
- |
- |
| Halalkalibacterium halodurans H4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein with His-tag | Halalkalibacterium halodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dynorphin + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
| dynorphin + H2O | - |
Halalkalibacterium halodurans H4 | ? | - |
? | |
| dynorphin A fragment 1-13 + H2O | cleavage at Phe4-Leu5, Leu5-Arg6, Arg6-Arg7, Arg7-Ile8 | Halalkalibacterium halodurans | ? | - |
? | |
| dynorphin A fragment 1-13 + H2O | cleavage at Phe4-Leu5, Leu5-Arg6, Arg6-Arg7, Arg7-Ile8 | Halalkalibacterium halodurans H4 | ? | - |
? | |
| Insulin B-chain + H2O | cleavage at Phe25-Tyr26, Tyr16-Leu17, His10-Leu11 | Halalkalibacterium halodurans | ? | - |
? | |
| Insulin B-chain + H2O | cleavage at Phe25-Tyr26, Tyr16-Leu17, His10-Leu11 | Halalkalibacterium halodurans H4 | ? | - |
? | |
| additional information | no substrate: 4-nitroanilides of several amino acids tested, azocasein, bovine serum albumin | Halalkalibacterium halodurans | ? | - |
? | |
| additional information | no substrate: 4-nitroanilides of several amino acids tested, azocasein, bovine serum albumin | Halalkalibacterium halodurans H4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 46500, SDS-PAGE, 1 * 46585, calculated | Halalkalibacterium halodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ppBH4 | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Halalkalibacterium halodurans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, stable | Halalkalibacterium halodurans |
| 50 | - |
half-life is 20 min | Halalkalibacterium halodurans |
| 55 | - |
half-life is 10 min | Halalkalibacterium halodurans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
Insulin B-chain | pH 8.0, 37°C | Halalkalibacterium halodurans | |
| 0.55 | - |
dynorphin A fragment 1-13 | pH 8.0, 37°C | Halalkalibacterium halodurans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity in sodium phosphate buffer is 3- to 4fold higher than in veronal or Tris/HCl buffer | Halalkalibacterium halodurans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Halalkalibacterium halodurans | calculated | - |
5.4 |
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