Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | casein as substrate | Crotalus atrox | |
EDTA | casein as substrate | Crotalus atrox |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc metalloproteinase, 1 mol zinc/mol enzyme | Crotalus atrox | |
Zn2+ | essential for hemorrhagic and proteolytic activity | Crotalus atrox |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition, immunologically not related to hemorrhagic toxin a or c | Crotalus atrox |
64000 | - |
1 * 64000, Crotalus atrox, SDS-PAGE | Crotalus atrox |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Basement membranes surrounding capillaries + H2O | Crotalus atrox | produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crotalus atrox | - |
Western diamondback rattlesnake | - |
Purification (Comment) | Organism |
---|---|
from lyophilized crude venom | Crotalus atrox |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Crotalus atrox | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Basement membranes surrounding capillaries + H2O | produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver | Crotalus atrox | ? | - |
? | |
casein + H2O | - |
Crotalus atrox | ? | - |
? | |
Dimethylcasein + H2O | - |
Crotalus atrox | ? | - |
? | |
Fibrinogen + H2O | no coagulation | Crotalus atrox | Hydrolyzed fibrinogen | non-clottable products | ? | |
Fibrinogen + H2O | cleaves gamma-chain, Aalpha and Bbeta-chains remain intact | Crotalus atrox | Hydrolyzed fibrinogen | non-clottable products | ? | |
additional information | no hydrolysis of tosyl-L-Arg methyl ester or N-benzoyl-L-Arg ethyl ester | Crotalus atrox | ? | - |
? | |
Oxidized insulin B-chain + H2O | cleaves Val2-Asn3, Gln4-His5, Leu6-Cys7, H10-Leu11, Ala14-Leu15, Tyr16-Leu17 bonds | Crotalus atrox | Hydrolyzed oxidized insulin B-chain | several peptides | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | in the presence of 2-mercaptoethanol | Crotalus atrox |
monomer | 1 * 64000, Crotalus atrox, SDS-PAGE | Crotalus atrox |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Crotalus atrox |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 7.7 | Crotalus atrox |