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Literature summary for 3.4.24.39 extracted from

  • Doi, Y.; Lee, B.R.; Ikeguchi, M.; Ohoba, Y.; Ikoma, T.; Tero-Kubota, S.; Yamauchi, S.; Takahashi, K.; Ichishima, E.
    Substrate specificities of deuterolysin from Aspergillus oryzae and electron paramagnetic resonance measurement of cobalt-substituted deuterolysin (2003), Biosci. Biotechnol. Biochem., 67, 264-270.
    View publication on PubMed
Search for more literature for 3.4.24.39

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0119
-
 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.0237
-
 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.0264
-
 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.0275
-
 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.0473
-
 Z-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.0522
-
 Z-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.0638
-
 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.07
-
 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.146
-
 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.1573
-
 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-protease, contains one mole of zinc per mole of enzyme Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
-
 Aspergillus oryzae
19020
-
 amino acid sequence Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
calf thymus histone 4 + H2O
-
 Aspergillus oryzae ?
-
 ?
additional information almost no hydrolysis toward mono or certain dibasic peptide 4-methylcoumarin-7-amides like N-butoxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Ile-Glu-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gln-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Leu-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Leu-Thr-Arg-4-methylcoumarin-7-amide, Z-Phe-Arg-4-methylcoumarin-7-amide, Arg-4-methylcoumarin-7-amide, Lys-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gly-Arg-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gln-Arg-Arg-4-methylcoumarin-7-amide, and N-butoxycarbonyl-Gln-Lys-Lys-4-methylcoumarin-7-amide Aspergillus oryzae ?
-
 ?
N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?
Z-Arg-Arg-4-methylcoumarin-7-amide + H2O
-
 Aspergillus oryzae ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 100 extremely stable at 100°C, but unstable near 75°C because of self-digestion Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00065
-
 Z-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.00082
-
 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.0009
-
 Z-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.0017
-
 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.0022
-
 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.0035
-
 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.0045
-
 N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.009
-
 N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae
0.017
-
 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide pH 9.0, 30°C, Co-substituted deuterolysin Aspergillus oryzae
0.022
-
 N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide pH 9.0, 30°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aspergillus oryzae