Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | possible autocatalytic mechanism for activation | Leishmania sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Benzyloxycarbonyl-Tyr-Leu-NHOH | i.e. hydroxamate-derivatized dipeptide, strong, azocasein as substrate | Leishmania sp. | |
additional information | no inhibition by carboxymethyl derivative of Phe-Leu or hydroxamate derivatives of Leu or Tyr | Leishmania sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr | - |
Leishmania sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Leishmania sp. | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc containing metalloproteinase | Leishmania sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Arg-Ser-Val-Val-Arg-Asp-Val-Asn + H2O | i.e. synthetic peptide derived from leishmanolysin | Leishmania sp. | Ala-Arg-Ser-Val + Val-Arg-Asp-Val-Asn | - |
? | |
Ala-Arg-Ser-Val-Val-Arg-Asp-Val-Asn + H2O | covers the last 4 amino acids of the prosequence and the first 5 amino acids of the native Leishmanolysin, cleavage site: Val-Val | Leishmania sp. | Ala-Arg-Ser-Val + Val-Arg-Asp-Val-Asn | - |
? | |
azocasein + H2O | - |
Leishmania sp. | low molecular weight acid-soluble azopeptides | - |
? | |
Glucagon + H2O | major cleavage sites: Tyr10-Ser11, Asp15-Ser16, minor site: Trp25-Leu26 | Leishmania sp. | ? | - |
? | |
Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr + H2O | from horse cytochrome | Leishmania sp. | Leu-Ile-Ala-Tyr + ? | - |
? | |
Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr + H2O | model peptide cytochrome c(94-102)L, cleavage site: Tyr97-Leu98 | Leishmania sp. | Leu-Ile-Ala-Tyr + ? | - |
? | |
Leu-Ile-Ala-Tyr-Ser-Lys-Lys-Ala-Thr + H2O | from horse cytochrome | Leishmania sp. | ? | - |
? | |
Leu-Ile-Ala-Tyr-Ser-Lys-Lys-Ala-Thr + H2O | model peptide cytochrome c(94-102)S, cleavage site: Tyr97-Ser98 | Leishmania sp. | ? | - |
? | |
additional information | substrate specificity | Leishmania sp. | ? | - |
? | |
additional information | preferentially cleaves at amino side when hydrophobic residues are in P1'-site, basic amino acids in P2' and P3' and commonly Tyr at P1 | Leishmania sp. | ? | - |
? | |
Oxidized insulin B-chain + H2O | major cleavage site: Gly8-Ser9, minor site: Tyr16-Leu17 | Leishmania sp. | ? | - |
? | |
Peptide 170-182 from CW-3 HLA antigen + H2O | major cleavage site: Lys75-Asn76, minor site: Tyr73-Leu74 | Leishmania sp. | ? | - |
? | |
Peptide 83-94 from beta-chain of HLA DO + H2O | cleavage site: Arg84-Leu85 | Leishmania sp. | ? | - |
? | |
Peptide 97-105 from Leishmanolysin + H2O | - |
Leishmania sp. | ? | - |
? | |
Peptides from horse cytochrome c + H2O | peptide 81-104 cleavage sites: Gly84-Ile85 and Tyr97-Leu98 | Leishmania sp. | ? | - |
? | |
Peptides from horse cytochrome c + H2O | i.e. peptides 1-25, 39-53 and 81-104 | Leishmania sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Promastigote surface protease | - |
Leishmania sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr as substrate | Leishmania sp. |
7.5 | 10 | azocasein as substrate | Leishmania sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.7 | 11.7 | about half-maximal activity at pH 4.7 and 11.7, azocasein as substrate | Leishmania sp. |
5.3 | 9.5 | about half-maximal activity at pH 5.3 and 9.5, Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr as substrate | Leishmania sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | at least 30 min stable | Leishmania sp. |