Cloned (Comment) | Organism |
---|---|
recombinant expression of the wild-type and mutant zymogen in Spodoptera frugiperda Sf9 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of the catalytically inactive mutant of zymogen proMMP-9, proMMP-9 MutE | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tissue inhibitor of metalloproteinases 1 | TIMP-1, the zymogen proMMP-9 trimers possess a 50fold higher affinity for TIMP-1 than the monomers, with a higher extent of TIMP-1 inhibition of angiogenesis induced by trimers compared with monomers | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | enzyme contains a Zn2+ -binding domain | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89300 | - |
x * 89300, about, SDS-PAGE, the natural zymogen proMMP-9 occurs as monomers, homomultimers and heterocomplexes. MMP-9 homomultimers are reduction-sensitive trimers, three-dimensional structure molecular modeling, overview. Homomultimerization of the enzyme involves cysteine bridge formation. The zymogen proMMP-9 monomer includes the propeptide, the fibronectin-like domain, the Zn2+ -binding domain, the catalytic domain, the flexible OG domain, and the haemopexin-like domain | Homo sapiens |
228300 | - |
enzyme multimer/trimer, PAGE, structure analysis by AFM | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
additional information | Homo sapiens | the enzyme cleaves many substrates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14780 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is produced by most cell types as a zymogen, proMMP-9 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant zymogen from Sf9 insect cells by gelatin affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | - |
Homo sapiens | - |
neutrophil | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | the enzyme cleaves many substrates | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 89300, about, SDS-PAGE, the natural zymogen proMMP-9 occurs as monomers, homomultimers and heterocomplexes. MMP-9 homomultimers are reduction-sensitive trimers, three-dimensional structure molecular modeling, overview. Homomultimerization of the enzyme involves cysteine bridge formation. The zymogen proMMP-9 monomer includes the propeptide, the fibronectin-like domain, the Zn2+ -binding domain, the catalytic domain, the flexible OG domain, and the haemopexin-like domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
gelatinase B | - |
Homo sapiens |
MMP-9 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme cleaves many substrates and is produced by most cell types as a zymogen, proMMP-9, in complex with the tissue inhibitor of metalloproteinases-1, TIMP-1, molecular modelling, overview | Homo sapiens |