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Literature summary for 3.4.24.33 extracted from
- Peptidyl-Asp metalloendopeptidase (1995), Methods Enzymol., 248, 782-787.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | slight activation at 5-10% v/v, inhibits at 1% | Pseudomonas fragi |  |
General Stability
| General Stability | Organism |
|---|---|
| Stable in up to 2 M urea, 0.01% w/v SDS or 0.1 M guanidine hydrochloride | Pseudomonas fragi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | Zn2+ does not restore after prolonged incubation | Pseudomonas fragi | |
| acetonitrile | 1% v/v, activation at 5-10% v/v | Pseudomonas fragi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas fragi | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | data obtained from inhibition experiments support metalloproteinase character | Pseudomonas fragi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
partial amino acid sequences | Pseudomonas fragi |
| 27000 | - |
Pseudomonas fragi, HPLC gel filtration | Pseudomonas fragi |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| acetonitrile | stable in 5-10% v/v acetonitrile | Pseudomonas fragi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fragi | - |
mutant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to near homogeneity | Pseudomonas fragi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Pseudomonas fragi | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, lyophilized in the presence of Tris-HCl buffer, pH 7.5, at least 2 years, 4 mg lyophilizate in 100 ml H2O, 2 weeks | Pseudomonas fragi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-tubulin + H2O | specific for preaspartate cleavage | Pseudomonas fragi | ? | - |
? | |
| Azocoll + H2O | - |
Pseudomonas fragi | ? | - |
? | |
| casein + H2O | resorufin-labeled casein | Pseudomonas fragi | ? | - |
? | |
| casein + H2O | specific for preaspartate cleavage | Pseudomonas fragi | ? | - |
? | |
| Glucagon + H2O | specific for preaspartate cleavage | Pseudomonas fragi | ? | - |
? | |
| Hemoglobin + H2O | - |
Pseudomonas fragi | ? | - |
? | |
| Insulin A-chain + H2O | - |
Pseudomonas fragi | ? | - |
? | |
| Insulin B-chain + H2O | specific for preaspartate cleavage | Pseudomonas fragi | ? | - |
? | |
| Insulin B-chain + H2O | also cleaves bonds with cysteic acid in P1' derived from cysteine residues by oxidation with performic acid and at N-terminal side of some glutamyl residues | Pseudomonas fragi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000, Pseudomonas fragi, SDS-PAGE | Pseudomonas fragi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas fragi |
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