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Literature summary for 3.4.24.32 extracted from

  • Kessler, E.
    Beta-lytic metalloendopeptidase (2004), Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 998-1000.
No PubMed abstract available

Application

Application Comment Organism
additional information ability to lyse Arthrobacter globiformis, Micrococcus luteus and Staphylococcus aureus cells, inhibits growth of sensitive organisms and may potentially serve as an antimicrobial agent Lysobacter enzymogenes
additional information ability to lyse Micrococcus luteus and Staphylococcus aureus cells Achromobacter lyticus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Lysobacter enzymogenes

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Lysobacter enzymogenes
additional information insensitive to DFP Lysobacter enzymogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required for activity Achromobacter lyticus
Zn2+ required for activity Lysobacter enzymogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19100
-
-
Lysobacter enzymogenes
19300
-
-
Achromobacter lyticus

Organism

Organism UniProt Comment Textmining
Achromobacter lyticus
-
-
-
Lysobacter enzymogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Achromobacter lyticus
-
Lysobacter enzymogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Lysobacter enzymogenes ?
-
?
elastin-orcein + H2O
-
Lysobacter enzymogenes ?
-
?
furylacryloyl-Gly-Leu-NH2 + H2O
-
Lysobacter enzymogenes ?
-
?
Insulin B-chain + H2O
-
Lysobacter enzymogenes ?
-
?
additional information no cleavage of Z-Gly-Phe Lysobacter enzymogenes ?
-
?
additional information the enzyme only cleaves Gly-X bonds and favors hydrophobic or apolar residues to either side, it does not hydrolyze bonds with charged amino acids or proline adjacent, cleaves D-Ala-Gly and D-Ala-Ala bonds in the linkage between peptide subunit and the interpeptide bridge and also the Gly-Gly bond in the Staphylococcus aureus interpeptide bridge Achromobacter lyticus ?
-
?
peptidoglycan + H2O
-
Achromobacter lyticus ?
-
?
peptidoglycan + H2O
-
Lysobacter enzymogenes ?
-
?
Z-Gly-Phe-NH2 + H2O
-
Lysobacter enzymogenes ?
-
?

Synonyms

Synonyms Comment Organism
beta-Lytic protease
-
Achromobacter lyticus
beta-Lytic protease
-
Lysobacter enzymogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
for FA-Gly-Leu-NH2 hydrolysis Lysobacter enzymogenes
9
-
for bacteriolysis Lysobacter enzymogenes