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Literature summary for 3.4.24.3 extracted from

  • Itoi, Y.; Horinaka, M.; Tsujimoto, Y.; Matsui, H.; Watanabe, K.
    Characteristic features in the structure and collagen-binding ability of a thermophilic collagenolytic protease from the thermophile Geobacillus collagenovorans MO-1 (2006), J. Bacteriol., 188, 6572-6579.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and anaylsis, sequence comparison, overexpression in Escherichia coli strains DH5alpha and BL21(DE3), overexpression of various regions of the collagenolytic protease as GST-tagged proteins Geobacillus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Geobacillus sp.
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Type I collagen + H2O Geobacillus sp. degradation ?
-
?
Type I collagen + H2O Geobacillus sp. MO-1 degradation ?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus sp. Q0KK26
-
-
Geobacillus sp. MO-1 Q0KK26
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme fragments, e.g. GST-Cat, a fusion protein with the catalytic region of the enzyme, by glutathione affinity chromatography to homogeneity Geobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
collagen-binding analysis, assay method, the three nonionic detergents, Triton X-100, Tween 20, and Tween 60, are ineffective for the release of the collagenolytic protease from collagen, overview Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Type I collagen + H2O degradation Geobacillus sp. ?
-
?
Type I collagen + H2O degradation Geobacillus sp. MO-1 ?
-
?

Subunits

Subunits Comment Organism
More the enzyme consists of a signal sequence for secretion, a prosequence for maturation, a catalytic region, 14 direct repeats of 20 amino acids at the C terminus, and a region with unknown function intervening between the catalytic region and the numerous repeats Geobacillus sp.

Synonyms

Synonyms Comment Organism
More the enzyme is a member of the subtilisin family of serine proteases Geobacillus sp.
thermophilic collagenolytic protease
-
Geobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Geobacillus sp.