Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and anaylsis, sequence comparison, overexpression in Escherichia coli strains DH5alpha and BL21(DE3), overexpression of various regions of the collagenolytic protease as GST-tagged proteins | Geobacillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Geobacillus sp. | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Type I collagen + H2O | Geobacillus sp. | degradation | ? | - |
? | |
Type I collagen + H2O | Geobacillus sp. MO-1 | degradation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | Q0KK26 | - |
- |
Geobacillus sp. MO-1 | Q0KK26 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme fragments, e.g. GST-Cat, a fusion protein with the catalytic region of the enzyme, by glutathione affinity chromatography to homogeneity | Geobacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
collagen-binding analysis, assay method, the three nonionic detergents, Triton X-100, Tween 20, and Tween 60, are ineffective for the release of the collagenolytic protease from collagen, overview | Geobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Type I collagen + H2O | degradation | Geobacillus sp. | ? | - |
? | |
Type I collagen + H2O | degradation | Geobacillus sp. MO-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme consists of a signal sequence for secretion, a prosequence for maturation, a catalytic region, 14 direct repeats of 20 amino acids at the C terminus, and a region with unknown function intervening between the catalytic region and the numerous repeats | Geobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the subtilisin family of serine proteases | Geobacillus sp. |
thermophilic collagenolytic protease | - |
Geobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Geobacillus sp. |