Literature summary for 3.4.24.3 extracted from

Eckhard, U.; Nuess, D.; Ducka, P.; Schoenauer, E.; Brandstetter, H.
Crystallization and preliminary X-ray characterization of the catalytic domain of collagenase G from Clostridium histolyticum (2008), Acta Crystallogr. Sect. F, 64, 419-421.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene colG, expression of the catalytic domain of N-terminally His-tagged, separated from ColG by a TEV cleavage site, collagenase G in Escherichia coli strain BL21 (DE3)	Hathewaya histolytica

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged catalytic domain of collagenase G, sitting drop vapour diffusion method, 10 mg/ml protein in 25 mM Tris, pH 7.5, and 50 mM NaCl, mixed with reservoir solution containing 0.12 M sodium citrate and 23% v/v PEG 3350 at 20°C, 10 days, X-ray diffraction structure determination and anaylsis at 2.75 A resolution	Hathewaya histolytica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the catalytic domain contains the consensus motif HEXXH that harbours the catalytic Zn2+ ion, in contrast to the zinc ligation in metzincins such as MMPs, bacterial collagenases are believed to employ glutamate as a third zinc ligand	Hathewaya histolytica

Organism

Organism	UniProt	Comment	Textmining
Hathewaya histolytica	Q9X721	gene colG	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant catalytic domain of His-tagged collagenase G from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to about 95% purity	Hathewaya histolytica

Subunits

Subunits	Comment	Organism
More	ColG possesses a single PKD-like domain and a duplicated CBD domain in addition to the catalytic domain, comprising residues Tyr119-Ala790	Hathewaya histolytica

Synonyms

Synonyms	Comment	Organism
ColG	-	Hathewaya histolytica
collagenase G	-	Hathewaya histolytica

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Release 2023.1 (January 2023)