Literature summary for 3.4.24.3 extracted from

Wilson, J.J.; Matsushita, O.; Okabe, A.; Sakon, J.
A bacterial collagen-binding domain with novel calcium-binding motif controls domain orientation (2003), EMBO J., 22, 1743-1752.

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, structure of the collagen-binding domain with an N-terminal domain linker from class I collagenase, determined at 1.0 A resolution in the absence of Ca2+ and at 1.65 A resolution in the presence of calcium	Hathewaya histolytica

Protein Variants

Protein Variants	Comment	Organism
D904A	mutant enzyme with increased MW in presence of Ca2+ and decreased MW in presence of 1 mM EGTA, determined by gel filtration	Hathewaya histolytica
D927A	mutant enzyme with increased MW in presence of Ca2+ and slightly decreased MW in presence of 1 mM EGTA, determined by gel filtration	Hathewaya histolytica
D930A	mutant enzyme with increased MW in presence of Ca2+ and slightly decreased MW in presence of 1 mM EGTA, determined by gel filtration	Hathewaya histolytica
E899A	mutant enzyme with increased MW in presence of Ca2+ and decreased MW in presence of 1 mM EGTA, determined by gel filtration	Hathewaya histolytica
E901A	mutant enzyme with increased MW in presence of Ca2+ and decreased MW in presence of 1 mM EGTA, determined by gel filtration	Hathewaya histolytica
N903A	mutant enzyme with increased MW in presence of Ca2+ and slightly increased MW in presence of 1 mM EGTA, determined by gel filtration	Hathewaya histolytica
Q972A	mutant enzyme with slightly decreased MW in presence of Ca2+	Hathewaya histolytica
S906A	mutant enzyme with slightly decreased MW in presence of Ca2+	Hathewaya histolytica
S906F	mutant enzyme with slightly decreased MW in presence of Ca2+ ad slightly increased MW in presence of 1 mM EGTA, determined by gel filtration	Hathewaya histolytica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	conformational change of the linker upon calcium binding in the structure of the collagen-binding domain with an N-terminal domain linker from class I collagenase	Hathewaya histolytica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
11470	-	mutant enzyme S906F in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
11750	-	mutant enzyme S906A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
11810	-	mutant enzyme Q972A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
11910	-	mutant enzyme V973A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
11920	-	wild-type enzyme in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
13720	-	mutant enzyme E901A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
13750	-	mutant enzyme D904A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
14390	-	mutant enzyme E899A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
15370	-	mutant enzyme D930A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
15420	-	mutant enzyme D930A in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica
15730	-	mutant enzyme N903A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
15790	-	mutant enzyme D927A in presence of 1 mM Ca2+, gel filtration	Hathewaya histolytica
15890	-	mutant enzyme E899A in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica
16160	-	mutant enzyme D904A in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica
16190	-	mutant enzyme D927A in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica
16410	-	mutant enzyme S906A in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica
16430	-	wild-type enzyme in presence of 1 mM EGTA, gel filtration	Hathewaya histolytica
16470	-	mutant enzyme Q972A in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica
16510	-	mutant enzyme V973A in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica
16530	-	mutant enzyme N903A in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica
16650	-	mutant enzyme S906F in presence of 1 mM EDTA, gel filtration	Hathewaya histolytica

Organism

Organism	UniProt	Comment	Textmining
Hathewaya histolytica	-	class I collagenase	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the N-terminal region of the collagen-binding region is not an essential structural component for collagen binding or recognition but rather a cation-controlled multiconformational domain-linker	Hathewaya histolytica	?	-	?