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Literature summary for 3.4.24.3 extracted from

  • Kono, T.
    Purification and partial characterization of collagenolytic enzymes from Clostridium histolyticum (1968), Biochemistry, 7, 1106-1114.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Hathewaya histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Hathewaya histolytica degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
?

Organism

Organism UniProt Comment Textmining
Hathewaya histolytica
-
3 forms: A-alpha, B-alpha and B-beta differ in substrate specificity
-

Purification (Commentary)

Purification (Comment) Organism
-
Hathewaya histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Hathewaya histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg + H2O
-
Hathewaya histolytica 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
-
?
Azocoll + H2O
-
Hathewaya histolytica Hydrolyzed azocoll
-
?
Collagen + H2O denatured Hathewaya histolytica Hydrolyzed collagen
-
?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Hathewaya histolytica Hydrolyzed collagen
-
?
Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Hathewaya histolytica ?
-
?