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Literature summary for 3.4.24.28 extracted from

  • Mansfeld, J.; Ulbrich-Hofmann, R.
    The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents (2007), Biotechnol. Bioeng., 97, 672-679.
    View publication on PubMed
Search for more literature for 3.4.24.28

Protein Variants

Protein Variants Comment Organism
G8C/N60C double mutant temperature optimum at 80°C, increased thermal stability Geobacillus stearothermophilus

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 48 h, 37°C, 50% residual activity with 44.5% (v/v) and 65.5% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
Acetone 48 h, 37°C, 50% residual activity with 30.0% (v/v) and 50.1% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
acetonitrile 48 h, 37°C, 50% residual activity with 30.6% (v/v) and 51.6% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
dimethylformamide 48 h, 37°C, 50% residual activity with 29.8% (v/v) and 45.8% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
dioxane 48 h, 37°C, 50% residual activity with 32.1% (v/v) and 47.9% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
DMSO 48 h, 37°C, 50% residual activity with 32.9% (v/v) and 48.7% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus
Methanol 48 h, 37°C, 50% residual activity with 56.6% (v/v) and 86.4% (v/v) for pseudo wild-type and mutant G8C/N60C respectively Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
78.5
-
 Boilysin at 37°C with casein as substrate Geobacillus stearothermophilus
81.5
-
 pseudo wild type at 37°C with casein as substrate Geobacillus stearothermophilus
81.9
-
 wild type enzyme at 37°C with casein as substrate Geobacillus stearothermophilus
82.4
-
 double mutant G8C/N60C at 37°C with casein as substrate Geobacillus stearothermophilus
242
-
 wild type enzyme at temperature optimum 75°C Geobacillus stearothermophilus
273
-
 pseudo wild type at temperature optimum 75°C Geobacillus stearothermophilus
375
-
 double mutant G8C/N60C at temperature optimum 80°C Geobacillus stearothermophilus
608
-
 boilysin at temperature optimum 95°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(2-furylacryloyl)-glycyl-L-leucinamide + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
casein + H2O
-
 Geobacillus stearothermophilus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 wild type enzyme Geobacillus stearothermophilus
75
-
 pseudo wild type Geobacillus stearothermophilus
80
-
 double mutant G8C/N60C Geobacillus stearothermophilus
95
-
 boilysin Geobacillus stearothermophilus