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Literature summary for 3.4.24.28 extracted from
- An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding (2005), FEBS J., 272, 1523-1534.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G8C/N60C | introduction of disulfide bridge fixing the region near calcium binding site III. At guanidinium/HCl concentrations above 7.5 M, mutant unfolds distinctly more slowly than wild-type. Below 7.5 M guanidinium/HCl, mutant behaves similar to wild-type | Geobacillus stearothermophilus |
| W55F | higher Ca2+ concentration is needed than for wild-type for occupation of Ca2+-binding site III at amino acids 55-59 | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminobenzoyl-AGLA-4-nitrobenzylamide + H2O | - |
Geobacillus stearothermophilus | ? | - |
? |  |
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Release 2023.1 (January 2023)
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