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Literature summary for 3.4.24.27 extracted from

  • Chen, F.; Zhang, F.; Du, F.; Wang, A.; Gao, W.; Wang, Q.; Yin, X.; Xie, T.
    A novel and efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation (2012), Biores. Technol., 115, 158-163.
    View publication on PubMed
Search for more literature for 3.4.24.27

Activating Compound

Activating Compound Comment Organism Structure
additional information microwave power output increases the level of activity of the immobilized thermolysin by 4.5fold, optimally at 40 W, overview Bacillus thermoproteolyticus

Protein Variants

Protein Variants Comment Organism
additional information evaluation of an efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation, overview. 4.6% of the relative activity for the immobilized thermolysin is detected when the immobilization mixture contains no salts, including ZnCl2, CaCl2 or NaCl Bacillus thermoproteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ CaCl2 does not exert any significant influence on the activity of immobilized thermolysin, but stabilizes the enzyme in absence of Zn2+ Bacillus thermoproteolyticus
NaCl activates at 3 M Bacillus thermoproteolyticus
Zn2+ a zinc metalloprotease Bacillus thermoproteolyticus

Organic Solvent Stability

Organic Solvent Comment Organism
2-methyl-2-butanol no reduction in activity of immobilized thermolysin after 2 h in 5% tert-amyl alcohol, whereas the free enzyme loses 32.5% of its catalytic activity Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
-
-
Bacillus thermoproteolyticus Rokko
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bacillus thermoproteolyticus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?
casein + H2O
-
 Bacillus thermoproteolyticus Rokko ?
-
 ?
N-carbobenzoxy-L-Asp-L-Phe methyl ester + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?
N-carbobenzoxy-L-Asp-L-Phe methyl ester + H2O
-
 Bacillus thermoproteolyticus Rokko ?
-
 ?

Synonyms

Synonyms Comment Organism
Neutral metalloproteinase
-
 Bacillus thermoproteolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bacillus thermoproteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bacillus thermoproteolyticus