Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.27 extracted from

  • Eijsink, V.; Matthews, B.; Vriend, G.
    The role of calcium ions in the stability and instability of a thermolysin-like protease (2011), Protein Sci., 20, 1346-1355.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.27

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol inactive pre-proprotein Geobacillus stearothermophilus 5829
-
extracellular the enzyme is secreted Geobacillus stearothermophilus
-
-
additional information the protein is translocated through the membrane at the expense of ATP and the pre-peptide is cleaved off by a type I signal peptidase Geobacillus stearothermophilus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ protease binds calcium ions in the regions that are involved in the autolytic maturation process, at least one of the calcium ions plays a regulatory role. This calcium ion plays an important role as a switch that modulates the protease between stable and unstable states as appropriate to the biological need Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification autolytic processing. Secreted proteases are produced as prepro-proteins. The pre-peptide is cleaved-off during Sec-controlled secretion, and the active protease emerges outside the cell after folding of the proprotein and autolytic removal of the pro-peptide. The protein is translocated through the membrane at the expense of ATP and the pre-peptide is cleaved off by a type I signal peptidase. The pro-part plays two roles in this process: it facilitates folding by acting as an intra-molecular chaperone and it inhibits protease activity of the folded pro-enzyme Geobacillus stearothermophilus

Subunits

Subunits Comment Organism
More TLPs have a two domain structure Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
thermolysin-like protease
-
 Geobacillus stearothermophilus
TLP
-
 Geobacillus stearothermophilus

General Information

General Information Comment Organism
additional information life cycle of the thermolysin-like protease from Bacillus stearothermophilus in light of the calcium-dependent stability and instability of the N-terminal domain. Geobacillus stearothermophilus