Crystallization (Comment) | Organism |
---|---|
three times crystallized and lyophilized preparation, Lot T5CB491 | Bacillus thermoproteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butanol | - |
Bacillus thermoproteolyticus | |
1-Pentanol | - |
Bacillus thermoproteolyticus | |
1-propanol | - |
Bacillus thermoproteolyticus | |
2-butanol | - |
Bacillus thermoproteolyticus | |
2-methyl-1-propanol | - |
Bacillus thermoproteolyticus | |
2-propanol | - |
Bacillus thermoproteolyticus | |
ethanol | - |
Bacillus thermoproteolyticus | |
methanol | - |
Bacillus thermoproteolyticus | |
tert-amyl alcohol | - |
Bacillus thermoproteolyticus | |
tert-amylalcohol | - |
Bacillus thermoproteolyticus | |
tert-butyl alcohol | - |
Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 4 calcium ions required for structural stability | Bacillus thermoproteolyticus | |
NaCl | induces activation | Bacillus thermoproteolyticus | |
Zn2+ | required for enzyme activity | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl) acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 + H2O | fluorescent substrate | Bacillus thermoproteolyticus | ? | - |
? | |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu-NH2 + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
1-Pentanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
38 | - |
1-propanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
40 | - |
2-butanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
41 | - |
2-methyl-1-propanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
50 | - |
1-butanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
65 | - |
1-propanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
100 | - |
ethanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
110 | - |
tert-amylalcohol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
190 | - |
tert-butyl alcohol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
430 | - |
methanol | pH 7.5, 25°C | Bacillus thermoproteolyticus |