Any feedback?
Literature summary for 3.4.24.26 extracted from
- Site-directed mutagenesis of Glu-141 and His-223 in Pseudomonas aeruginosa elastase: catalytic activity, processing, and protective activity of the elastase against Pseudomonas infection (1993), Infect. Immun., 61, 1400-1405.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the protective activity of elastase mutants against Pseudomonas infections may be useful in producing vaccines | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutants E141D, E141Q, E141G, H223G, H223E, and H223L in Escherichia coli JM109 | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E141D | no activity | Pseudomonas aeruginosa |
| E141G | no activity | Pseudomonas aeruginosa |
| E141Q | no activity | Pseudomonas aeruginosa |
| H223E | no activity | Pseudomonas aeruginosa |
| H223G | no activity | Pseudomonas aeruginosa |
| H223L | no activity | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | mutants E141D, E141Q, E141G, H223G, H223E, and H223L | Pseudomonas aeruginosa | 16020 | - |
| periplasm | wild-type | Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE, mature elastase | Pseudomonas aeruginosa |
| 51000 | - |
x * 51000, SDS-PAGE, precursor form of elastase | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | P14756 | IFO 3455 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | 37°C, pH 8.0 | Pseudomonas aeruginosa | ? | - |
? |  |
| elastin Congo red + H2O | pH 8.0 | Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE, mature elastase | Pseudomonas aeruginosa |
| ? | x * 51000, SDS-PAGE, precursor form of elastase | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pseudomonas aeruginosa elastase | - |
Pseudomonas aeruginosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
