Application | Comment | Organism |
---|---|---|
medicine | 2-mercaptoacetyl-L-phenylalanyl-L-leucine represents a class of potent elastase inhibitors that might prove useful in the management of Pseudomonas aeruginosa infections | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoacetyl-Leu-Dphe | - |
Pseudomonas aeruginosa | |
2-mercaptoacetyl-Leu-Phe | - |
Pseudomonas aeruginosa | |
2-mercaptoacetyl-Phe-Leu | at 0.1 mM 96% inhibition with azocasein as substrate, 97% inhibition with elastin as substrate and 97% inhibition with cartilage as substrate, at 0.01 mM 77% inhibition with azocasein as substrate, 33% inhibition with elastin as substrate and 66% inhibition with cartilage as substrate | Pseudomonas aeruginosa | |
benzyloxycarbonyl-Gly-NHOH | at 14 mM 98% inhibition with azocasein as substrate, 95% inhibition with elastin as substrate and 95% inhibition with cartilage as substrate, at 1.4 mM 77% inhibition with azocasein as substrate, 84% inhibition with elastin as substrate and 67% inhibition with cartilage as substrate | Pseudomonas aeruginosa | |
benzyloxycarbonyl-L-leucine | - |
Pseudomonas aeruginosa | |
benzyloxycarbonyl-L-phenylalanine | - |
Pseudomonas aeruginosa | |
benzyloxycarbonyl-Leu-NHOH | at 5.0 mM 98% inhibition with azocasein as substrate, 100% inhibition with elastin as substrate and 94% inhibition with cartilage as substrate, at 0.5 mM 76% inhibition with azocasein as substrate, 93% inhibition with elastin as substrate and 57% inhibition with cartilage as substrate | Pseudomonas aeruginosa | |
benzyloxycarbonyl-Phe-NHOH | - |
Pseudomonas aeruginosa | |
Leu-Phe | - |
Pseudomonas aeruginosa | |
Phe-Leu | - |
Pseudomonas aeruginosa | |
phosphoryl-L-leucyl-L-phenylalanine | at 0.1 mM 98% inhibition with azocasein as substrate, 94% inhibition with elastin as substrate and 98% inhibition with cartilage as substrate, at 0.01 mM 83% inhibition with azocasein as substrate, 87% inhibition with elastin as substrate and 89% inhibition with cartilage as substrate | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P14756 | Habs serotype 1 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C, 0.02 M Tris-hydrochloride, 0.5 mM CaCl2 (pH 7.5), 1 year, less than 10% loss of activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | pH 7.5, 37°C | Pseudomonas aeruginosa | ? | - |
? | |
cartilage + H2O | major components proteoglycans and collagen | Pseudomonas aeruginosa | ? | - |
? | |
Elastin + H2O | pH 7.5, 37°C | Pseudomonas aeruginosa | ? | - |
? | |
furylacryloyl-glycyl-L-leucyl-L-alanine + H2O | pH 7.5, 25°C | Pseudomonas aeruginosa | furylacryloyl-glycine + L-leucyl-L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
Pseudomonas aeruginosa elastase | - |
Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
phosphoryl-L-leucyl-L-phenylalanine | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
0.0002 | - |
2-mercaptoacetyl-Phe-Leu | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
0.0015 | - |
2-mercaptoacetyl-Leu-Phe | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
0.011 | - |
benzyloxycarbonyl-Leu-NHOH | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
0.021 | - |
benzyloxycarbonyl-Phe-NHOH | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
0.028 | - |
benzyloxycarbonyl-Gly-NHOH | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
0.034 | - |
2-mercaptoacetyl-Leu-Dphe | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
0.4 | - |
Phe-Leu | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
1.1 | - |
Leu-Phe | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
5 | - |
benzyloxycarbonyl-L-phenylalanine | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa | |
6.2 | - |
benzyloxycarbonyl-L-leucine | at pH 7.5 and 25°C, with furylacryloyl-glycyl-L-leucyl-L-alanine as substrate | Pseudomonas aeruginosa |