Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudoalteromonas sp. SM495 |
expressed in Escherichia coli BL21(DE3) cells | Pseudoalteromonas sp. SM9913 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. SM495 | B6E623 | - |
- |
Pseudoalteromonas sp. SM9913 | A1DRD5 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DEAE-Sepharose column chromatography | Pseudoalteromonas sp. SM495 |
ammonium sulfate precipitation and DEAE-Sepharose column chromatography | Pseudoalteromonas sp. SM9913 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(2-furylacryloyl)glycyl-L-leucine + H2O | - |
Pseudoalteromonas sp. SM495 | ? | - |
? | |
3-(2-furylacryloyl)glycyl-L-leucine + H2O | - |
Pseudoalteromonas sp. SM9913 | ? | - |
? | |
casein + H2O | - |
Pseudoalteromonas sp. SM495 | ? | - |
? | |
casein + H2O | - |
Pseudoalteromonas sp. SM9913 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
E495 | - |
Pseudoalteromonas sp. SM495 |
MCP-02 | - |
Pseudoalteromonas sp. SM9913 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
using casein as substrate, at pH 7.5 | Pseudoalteromonas sp. SM495 |
57 | - |
using casein as substrate, at pH 8.0 | Pseudoalteromonas sp. SM9913 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | - |
Pseudoalteromonas sp. SM495 |
40 | 70 | - |
Pseudoalteromonas sp. SM9913 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 64 | the melting temperature is at 64°C, the half-life at 55°C is 82 min, the half-life at 60°C is 20 min | Pseudoalteromonas sp. SM495 |
55 | 64 | the melting temperature is at 64°C, the half-life at 55°C is 90 min, the half-life at 60°C is 23 min | Pseudoalteromonas sp. SM9913 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
using casein as substrate, at 60°C | Pseudoalteromonas sp. SM495 |
8 | - |
using casein as substrate, at 60°C | Pseudoalteromonas sp. SM9913 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.32 | - |
3-(2-furylacryloyl)glycyl-L-leucine | at 25°C, at pH 8.0 | Pseudoalteromonas sp. SM9913 | |
2.55 | - |
3-(2-furylacryloyl)glycyl-L-leucine | at 25°C, at pH 7,5 | Pseudoalteromonas sp. SM495 |