Cloned (Comment) | Organism |
---|---|
recombinant expression of soluble and functional full-length enzyme from plasmid pET5a-MMP-2 in the cytoplasm of Escherichia coli strain BL21(DE3)/pLysS, subcloning in Escherichia coli strain DH5alpha, method development and evaluation, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted as a zymogen | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, zymogen, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
additional information | Homo sapiens | the proteinase has the capacity to degrade several extracellular matrix components such as type IV collagens as well as all nonfibrillar collagens, laminin, fibronectin, casein, elastin, and even fibrillar collagens | ? | - |
? | |
Type IV collagen + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08253 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is secreted as a zymogen and requires activation by cleavage of its propeptide | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme from Escherichia coli cell-free extract by gelatin affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
Gelatin + H2O | fluorescent substrate for use in the assay | Homo sapiens | ? | - |
? | |
additional information | the proteinase has the capacity to degrade several extracellular matrix components such as type IV collagens as well as all nonfibrillar collagens, laminin, fibronectin, casein, elastin, and even fibrillar collagens | Homo sapiens | ? | - |
? | |
Type IV collagen + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, zymogen, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
matrix metalloproteinase-2 | - |
Homo sapiens |
MMP-2 | - |
Homo sapiens |
Type IV collagenase | - |
Homo sapiens |